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A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

Medina-Carmona, Encarnacion, Varela, Lorena, Hendry, Alex C., Thompson, Gary S., White, Lisa J., Boles, Jessica E., Hiscock, Jennifer R., Ortega-Roldan, Jose L. (2020) A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chemical Communications, . ISSN 1359-7345. (In press) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:82527)

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Language: English

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Abstract

The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine \(^1H-^{31}P NMR\).

Item Type: Article
Subjects: Q Science
Q Science > Q Science (General)
Divisions: Faculties > Sciences > School of Biosciences
Faculties > Sciences > School of Physical Sciences
Depositing User: Jennifer Hiscock
Date Deposited: 20 Aug 2020 15:17 UTC
Last Modified: 25 Aug 2020 15:02 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/82527 (The current URI for this page, for reference purposes)
Thompson, Gary S.: https://orcid.org/0000-0001-9399-7636
Hiscock, Jennifer R.: https://orcid.org/0000-0002-1406-8802
Ortega-Roldan, Jose L.: https://orcid.org/0000-0002-6316-4390
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