Skip to main content
Kent Academic Repository

A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

Medina-Carmona, Encarnacion, Varela, Lorena, Hendry, Alex C., Thompson, Gary S., White, Lisa J., Boles, Jessica E., Hiscock, Jennifer R., Ortega-Roldan, Jose L. (2020) A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chemical Communications, 56 (78). pp. 11665-11668. ISSN 1359-7345. (doi:10.1039/D0CC03612A) (KAR id:82527)

PDF (Version of Record) Publisher pdf
Language: English


Download this file
(PDF/1MB)
[thumbnail of Version of Record]
Preview
Request a format suitable for use with assistive technology e.g. a screenreader
XML Word Processing Document (DOCX) Author's Accepted Manuscript
Language: English

Restricted to Repository staff only

Contact us about this Publication
[thumbnail of LipidSelectivity_Revised_final_minorRevisions.docx]
XML Word Processing Document (DOCX) Supplemental Material
Language: English


Download this file
(XML Word Processing Document (DOCX)/9MB)
[thumbnail of Supporting Information-Revised_finalRevisions.docx]
Request a format suitable for use with assistive technology e.g. a screenreader
Official URL:
https://doi.org/10.1039/D0CC03612A

Abstract

The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine \(^1H-^{31}P NMR\).

Item Type: Article
DOI/Identification number: 10.1039/D0CC03612A
Subjects: Q Science
Q Science > Q Science (General)
Divisions: Divisions > Division of Natural Sciences > Biosciences
Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Jennifer Hiscock
Date Deposited: 20 Aug 2020 15:17 UTC
Last Modified: 24 Feb 2023 09:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/82527 (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.