Seasonal variation in environmental DNA in relation to population size and environmental factors

Buxton, Andrew S., Groombridge, Jim J., Zakaria, Nurulhuda B., Griffiths, Richard A. (2017) Seasonal variation in environmental DNA in relation to population size and environmental factors. Scientific Reports, 7 . p. 46294. ISSN 2045-2322. (doi:10.1038/srep46294)

Abstract

Analysing DNA that organisms release into the environment (environmental DNA, or eDNA) has enormous potential for assessing rare and cryptic species. At present the method is only reliably used to assess the presence-absence of species in natural environments, as seasonal influences on eDNA in relation to presence, abundance, life stages and seasonal behaviours are poorly understood. A naturally colonised, replicated pond system was used to show how seasonal changes in eDNA were influenced by abundance of adults and larvae of great crested newts (Triturus cristatus). Peaks in eDNA were observed in early June when adult breeding was coming to an end, and between mid-July and mid- August corresponding to a peak in newt larval abundance. Changes in adult body condition associated with reproduction also influenced eDNA concentrations, as did temperature (but not rainfall or UV). eDNA concentration fell rapidly as larvae metamorphosed and left the ponds. eDNA concentration may therefore reflect relative abundance in different ponds, although environmental factors can affect the concentrations observed. Nevertheless, eDNA surveys may still represent an improvement over unadjusted counts which are widely used in population assessments but have unreliable relationships with population size.

Item Type:	Article
DOI/Identification number:	10.1038/srep46294
Subjects:	Q Science Q Science > QH Natural history Q Science > QH Natural history > QH426 Genetics Q Science > QH Natural history > QH541 Ecology Q Science > QH Natural history > QH75 Conservation (Biology) Q Science > QL Zoology
Divisions:	Faculties > Social Sciences > School of Anthropology and Conservation > DICE (Durrell Institute of Conservation and Ecology)
Depositing User:	Richard Griffiths
Date Deposited:	20 Apr 2017 15:56 UTC
Last Modified:	29 May 2019 18:58 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/61413 (The current URI for this page, for reference purposes)
Groombridge, Jim J.:	https://orcid.org/0000-0002-6941-8187
Griffiths, Richard A.:	https://orcid.org/0000-0002-5533-1013

Available versions of this item

• Seasonal variation in environmental DNA in relation to population size and environmentalfactors. (deposited 20 Apr 2017 15:47)
  • Seasonal variation in environmental DNA in relation to population size and environmental factors. (deposited 20 Apr 2017 15:56) [Currently Displayed]