Mapping of the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) to chicken chromosome 3

Ge, S F, Romanov, Michael N, Sharp, P J, Burt, David W, Paton, Ian R, Dunn, I C (2001) Mapping of the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) to chicken chromosome 3. Animal Genetics, 32 (1). p. 50. ISSN 0268-9146. (doi:10.1111/j.1365-2052.2001.0647i.pp.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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http://dx.doi.org/10.1111/j.1365-2052.2001.0647i.p...

Abstract

Source/description: The chicken luteinizing hormone/choriogonadotropin receptor gene has been cloned and sequenced. To produce markers for linkage mapping genomic DNA sequence from exon 7 to exon 9 including the intervening introns and exon was cloned and sequenced (EMBL accession number AJ289775). The intron-exon structure of this region, including exon 8 was the same as in mammalian LHCGR genes. A pair of primers for PCR which gave a single amplification product of 287 bp were then designed for the purposes of mapping. The sequence of the amplified product was confirmed by sequencing (97.6% sequence identity with AJ289775). Polymorphism was revealed by single strand conformation polymorphism (SSCP) analysis. Chromosomal location: Segregation analysis indicated that the chicken luteinizing hormone/choriogonadotropin receptor gene was located on chromosome 3, placing it at position 45.35. The marker for the luteinizing hormone receptor gene (ROS0250) is tightly linked (phi = 0.0) to MCW0169 and ADL0177 with lod scores of 14.4 and 15.7, respectively. There was no evidence for conservation of gene order in this region of chicken chromosome 3 with human or mouse chromosomes.

Item Type: Article
DOI/Identification number: 10.1111/j.1365-2052.2001.0647i.pp.x
Uncontrolled keywords: agriculture; genetics & heredity
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 12 Dec 2013 17:05 UTC
Last Modified: 29 May 2019 11:40 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37571 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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