Burden, Frances, Rathje, Claudia C., Ellis, Peter J.I., Holl, Justin, Lewis, Craig R.G., Farré, Marta (2025) Detecting chromosomal rearrangements in boars using Hi-C. Animal Genetics, 56 (2). Article Number e70009. ISSN 0268-9146. E-ISSN 1365-2052. (doi:10.1111/age.70009) (KAR id:110323)
|
PDF
Publisher pdf
Language: English 
This work is licensed under a Creative Commons Attribution 4.0 International License.
|
|
|
Download this file (PDF/1MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
| Official URL: https://doi.org/10.1111/age.70009 |
|
Abstract
A chromosomal rearrangement such as a reciprocal translocation (RT) in a breeding boar can produce unbalanced gametes during meiosis, leading to a decreased litter size with detrimental economic implications for breeders. FISH and standard karyotyping are currently used to detect RTs, but a fresh sample is required, limiting the shipping conditions. Here, we investigated Hi-C as an alternative technique to diagnose chromosome rearrangements. We show that Hi-C can be used to detect such RTs from either a fresh or a frozen blood sample and therefore this technique represents an alternative to FISH for RT detection.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1111/age.70009 |
| Uncontrolled keywords: | genotyping; chromosomal rearrangements; fertility; livestock; Hi-C |
| Subjects: |
Q Science > QH Natural history > QH324.2 Computational biology Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology (Living systems) > QP506 Molecular biology S Agriculture > SF Animal culture |
| Institutional Unit: | Schools > School of Natural Sciences > Biosciences |
| Former Institutional Unit: |
There are no former institutional units.
|
| Funders: | Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
| Depositing User: | Peter Ellis |
| Date Deposited: | 19 Jun 2025 13:06 UTC |
| Last Modified: | 22 Jul 2025 09:23 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/110323 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Burden, Frances.
| Creator's ORCID: |
 https://orcid.org/0000-0001-6266-1304
|
|---|---|
| CReDIT Contributor Roles: | Writing - original draft, Methodology, Investigation, Validation, Writing - review and editing, Formal analysis |
Rathje, Claudia C..
| Creator's ORCID: | |
|---|---|
| CReDIT Contributor Roles: | Validation, Investigation |
Ellis, Peter J.I..
| Creator's ORCID: |
https://orcid.org/0000-0001-9709-7934
|
|---|---|
| CReDIT Contributor Roles: | Writing - review and editing |
Farré, Marta.
| Creator's ORCID: |
https://orcid.org/0000-0001-9170-5767
|
|---|---|
| CReDIT Contributor Roles: | Writing - review and editing, Project administration, Supervision, Funding acquisition, Conceptualisation |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric
