Yildirim, Elena A., Ilina, Larisa A., Laptev, Georgi Yu., Tyurina, D G, Filippova, Valentina A., Dubrovin, Andrei V., Novikova, Natalia I., Kalitkina, Kseniya A., Djepbarova, Ogulgerek, Ponomareva, Ekaterina S., and others. (2024) The search for sources of enterobacteria and clostridia endotoxins in Russian dairy farms: possible transfer of endotoxins through the feed-cow-milk chain. In: Agriculture Digitalization and Organic Production. Proceedings of the Fourth International Conference on Agriculture Digitalization and Organic Production (ADOP 2024), Minsk, Belarus, June 05-08, 2024. Smart Innovation, Systems and Technologies , 397. pp. 325-335. Springer Nature Singapore Pte Ltd., Singapore ISBN 9789819744091 (Hardcover), 9789819744121 (Softcover). E-ISBN 978-981-97-4410-7. (doi:10.1007/978-981-97-4410-7_27) (KAR id:107906)
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| Official URL: https://doi.org/10.1007/978-981-97-4410-7_27 |
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Abstract
Here, samples to identify bacterial endotoxins were collected from two commercial dairy farms in Leningrad Oblast: farm A (samples of feces and milk were taken) and farm (where samples were taken from the feeding table, milk and rumen chyme). The study comprised four groups (A1, A2, B1, B2) where 1 was the control and 2 the test group. A1 were healthy, A2 showed signs of pathologies of the limb joints, B1 received the basic diet (BD) and B2 were fed this, plus the feed additive AntiKlos. Using PCR, samples were examined for the presence of genes for Shiga toxins (stx1A, stx2B), intimin (eae) and enterohemolysin (ehxA) produced by enterobacteria; for alpha (cpa1), beta (cpb) and epsilon toxin (etx) produced by Clostridium perfringens, plus binary toxin (cdtB), toxin A (tcdA) and toxin B (tcdB) produced by Cl. difficile. In the test Group A2, one animal out of six studied (16.7%) had the intimin (eae) and enterohemolysin (ehxA) genes produced by enterobacteria that were not found in the control A1. The epsilon toxin gene (etx) was the most common and present in 100% of the examined fecal samples from both farms and 100% of milk samples from farm A.
| Item Type: | Conference or workshop item (Paper) |
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| DOI/Identification number: | 10.1007/978-981-97-4410-7_27 |
| Projects: | Development of an Integrated Biotechnological Approach for the Biological Protection of Cattle and Livestock Products from Pathogenic Bacteria and Their Toxins |
| Uncontrolled keywords: | Dairy cows; Clostridia; Enterobacteria; Feed; Milk; Endotoxins |
| Subjects: |
Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology S Agriculture > SF Animal culture |
| Divisions: |
Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Mike Romanov |
| Date Deposited: | 23 Nov 2024 09:28 UTC |
| Last Modified: | 23 Nov 2024 09:28 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/107906 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Griffin, Darren K..
| Creator's ORCID: |
 https://orcid.org/0000-0001-7595-3226
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| CReDIT Contributor Roles: |
Romanov, Michael N.
| Creator's ORCID: |
https://orcid.org/0000-0003-3584-4644
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| CReDIT Contributor Roles: |
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