Effects of glyphosate and antibiotics on the expression of genes related to performance, antioxidant protection and histological barrier in the cecum of broilers

Laptev, Georgi Yu., Tyurina, D G, Yildirim, Elena A., Gorfunkel, Elena P., Ilina, Larisa A., Filippova, Valentina A., Dubrovin, Andrei V., Dubrovina, Alisa S., Novikova, Natalia I., Melikidi, Veronika Kh., and others. (2024) Effects of glyphosate and antibiotics on the expression of genes related to performance, antioxidant protection and histological barrier in the cecum of broilers. In: Agriculture Digitalization and Organic Production. Proceedings of the Fourth International Conference on Agriculture Digitalization and Organic Production (ADOP 2024), Minsk, Belarus, June 05-08, 2024. Smart Innovation, Systems and Technologies , 397. pp. 337-345. Springer Nature Singapore Pte Ltd., Singapore ISBN 9789819744091 (Hardcover), 9789819744121 (Softcover). E-ISBN 978-981-97-4410-7. (doi:10.1007/978-981-97-4410-7_28) (KAR id:107905)

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Official URL: https://doi.org/10.1007/978-981-97-4410-7_28

Abstract

In conditions of intensive poultry farming, significant amounts of xenobiotics enter the bird’s body. To investigate this, four groups of Ross 308 broiler chickens were formed: (1) control group fed the basic diet (BD); (2) experimental group fed BD supplemented with glyphosate; (3) experimental group fed BD along with combination of glyphosate and two antibiotics, enrofloxacin and colistin methanesulfonate. Analysis of the expression of genes for performance (IGF1, IGF2, MYOG, MYOZ2, SLC2A1, SLC2A2, SLC5A1, MSTN and TGFB1), antioxidant defense (CAT, SOD1, PRDX6 and HMOX1) and histological barrier function (MUC2, OCLN and CLDN1) in cecal tissues of birds were carried out using quantitative RT-PCR using a DTlight thermal cycler (DNA-Technology, Russia) and the SsoAdvancedTM Universal SYBR® Green Supermix kit (Bio-Rad, USA). The results showed that glyphosate alone (Group 2) inhibited the expression of a number of genes associated with productivity (IGF1, IGF2, SLC5A1 and MSTN) up to 4.1 times as compared with Group 1 (p < 0.05). In Groups 2 and 3, there was a decrease in almost all cases in the mRNA production of the MUC2, OCLN and CLDN1 genes in intestinal tissues from 1.3 to 2.2 times as compared to the control (p < 0.05).

Item Type:	Conference or workshop item (Paper)
DOI/Identification number:	10.1007/978-981-97-4410-7_28
Uncontrolled keywords:	Glyphosate; Antibiotics; Caeca of the intestine; Broilers; Gene expression
Subjects:	Q Science > QH Natural history > QH426 Genetics S Agriculture S Agriculture > SF Animal culture
Divisions:	Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Mike Romanov
Date Deposited:	23 Nov 2024 08:21 UTC
Last Modified:	23 Nov 2024 09:15 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/107905 (The current URI for this page, for reference purposes)

University of Kent Author Information

Griffin, Darren K..

Creator's ORCID:	https://orcid.org/0000-0001-7595-3226
CReDIT Contributor Roles:

Romanov, Michael N.

Creator's ORCID:	https://orcid.org/0000-0003-3584-4644
CReDIT Contributor Roles:

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