Analysis of changes in broiler microbiome biodiversity parameters due to intake of glyphosate and probiotic Bacillus sp. GL-8 using next-generation sequencing

Laptev, Georgi Yu., Turina, Darya G., Yildirim, Elena A., Ilina, Larisa A., Gorfunkel, Elena P., Filippova, Valentina A., Dubrovin, Andrei V., Melikidi, Veronika Kh., Novikova, Natalia I., Kalitkina, Kseniya A., and others. (2023) Analysis of changes in broiler microbiome biodiversity parameters due to intake of glyphosate and probiotic Bacillus sp. GL-8 using next-generation sequencing. In: Agriculture Digitalization and Organic Production. Proceedings of the Third International Conference on Agriculture Digitalization and Organic Production (ADOP 2023), St. Petersburg, Russia, June 05–07, 2023. Smart Innovation, Systems and Technologies , 362. pp. 161-170. Springer Nature Singapore Pte Ltd., Singapore ISBN 9789819941643 (Hardcover), 9789819941674 (Softcover). E-ISBN 978-981-99-4165-0. (doi:10.1007/978-981-99-4165-0_15) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:102452)

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Official URL: https://doi.org/10.1007/978-981-99-4165-0_15

Abstract

In recent years, there have been more data that the nonselective herbicide glyphosate (GLY) can negatively impact gut bacterial communities. The aim of our study was to investigate the composition of broiler caecal microbiome under chronic exposure to GLY and the introduction of a probiotic microorganism strain into the diet. 120 broilers were divided into three groups: Group 1 of control birds fed the basic diet (BD); Group 2 of experimental birds fed BD supplemented with GLY; and Group 3 of experimental birds fed BD supplemented with GLY and a probiotic strain of the microorganism Bacillus sp. GL-8. For analysis, we used the next-generation sequencing (NGS) technique. Due to the GLY administration, there was a trend of lowering the biodiversity of normal microflora representatives, along with intestinal colonization by undesirable forms of microorganisms. In particular, when adding GLY (Group 2), we observed a decreased number of Tepidimicrobium representatives (0.001 ±0.00006%) that ferment indigestible polysaccharides, while in Group 1 their content was greater (0.3 ± 0.02%; P ≤ 0.05). In Group 3 with probiotic, there was a lower number of Firmicutes (by 16.7%) and a rise in the number of Bacteroidetes (by 19.1%) as compared to Group 2 (P ≤ 0.05).

Item Type:	Conference or workshop item (Paper)
DOI/Identification number:	10.1007/978-981-99-4165-0_15
Uncontrolled keywords:	glyphosate, broilers, probiotic, Bacillus sp., microbiome, NGS sequencing
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology S Agriculture > SF Animal culture
Divisions:	Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction Divisions > Division of Natural Sciences > Biosciences
Signature Themes:	Food Systems, Natural Resources and Environment
Depositing User:	Mike Romanov
Date Deposited:	15 Aug 2023 11:25 UTC
Last Modified:	23 Nov 2024 08:54 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/102452 (The current URI for this page, for reference purposes)

University of Kent Author Information

Romanov, Michael N.

Creator's ORCID:	https://orcid.org/0000-0003-3584-4644
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