Effect of a probiotic strain administration in different feeding phases on α- and β-diversity and gene expression of the rumen microbiome in lactating cows

Laptev, Georgiy, Yildirim, Elena A., Ilina, Larisa A., Ponomareva, Ekaterina S., Kalitkina, Kseniya A., Turina, Darya G., Filippova, Valentina A., Dubrovin, Andrei V., Bashir, Khairullamin, Smetannikova, Tatyana, and others. (2023) Effect of a probiotic strain administration in different feeding phases on α- and β-diversity and gene expression of the rumen microbiome in lactating cows. In: Agriculture Digitalization and Organic Production. Proceedings of the Third International Conference on Agriculture Digitalization and Organic Production (ADOP 2023), St. Petersburg, Russia, June 05–07, 2023. Smart Innovation, Systems and Technologies , 362. pp. 181-191. Springer Nature Singapore Pte Ltd., Singapore ISBN 9789819941643 (Hardcover), 9789819941674 (Softcover). E-ISBN 978-981-99-4165-0. (doi:10.1007/978-981-99-4165-0_17) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:102447)

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Official URL: https://doi.org/10.1007/978-981-99-4165-0_17

Abstract

In cows, there is a drastic metabolic stress caused by violations of the rumen microbiome composition during lactation. The aim of the present study was to investigate the α-and β-diversity of the rumen microbiome of lactating cows using next-generation sequencing (NGS) and gene expression assessed by qPCR, as well as evaluation of the corrective properties of a probiotic strain introduced into the rumen. The results showed that the addition of the probiotic to the diet contributed to an effective increase in the fat content of milk during the early lactation period (P ≤ 0.05). The NGS-based analysis of the rumen microbiome of the studied cows using the 16S rRNA gene sequences showed that the Shannon and Chao1 indices of α-diversity of prokaryotic communities remained unchanged during various feeding phases, as well as due to the probiotic effects. In the early lactation period and under the probiotic’s impact, the number of representatives of the Clostridia_UCG-014 and Clostridiaceae families declined by 2.4 and 1.6 times, respectively (in Group 2 as compared to Group 1, P ≤ 0.05). Expression of the bacterial Ldh-L and ldhD genes was lower by 2.9 and 13.5 times, respectively (P ≤ 0.05), when adding the probiotic to the diet in the early lactation period.

Item Type:	Conference or workshop item (Paper)
DOI/Identification number:	10.1007/978-981-99-4165-0_17
Uncontrolled keywords:	Rumen microbiome; Gene expression; Probiotic; NGS sequencing; Lactating cows
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology S Agriculture > SF Animal culture
Divisions:	Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction Divisions > Division of Natural Sciences > Biosciences
Signature Themes:	Food Systems, Natural Resources and Environment
Depositing User:	Mike Romanov
Date Deposited:	15 Aug 2023 09:48 UTC
Last Modified:	05 Nov 2024 13:08 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/102447 (The current URI for this page, for reference purposes)

University of Kent Author Information

Romanov, Michael N.

Creator's ORCID:	https://orcid.org/0000-0003-3584-4644
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