Laptev, Georgiy, Yildirim, Elena A., Ilina, Larisa A., Ponomareva, Ekaterina S., Kalitkina, Kseniya A., Turina, Darya G., Filippova, Valentina A., Dubrovin, Andrei V., Bashir, Khairullamin, Smetannikova, Tatyana, and others. (2023) Effect of a probiotic strain administration in different feeding phases on α- and β-diversity and gene expression of the rumen microbiome in lactating cows. In: Agriculture Digitalization and Organic Production: Proceedings of the Third International Conference on Agriculture Digitalization and Organic Production (ADOP 2023). Smart Innovation, Systems and Technologies , 362. pp. 181-191. Springer Nature Singapore Pte Ltd., Singapore ISBN 9789819941643 (Hardcover), 9789819941674 (Softcover). E-ISBN 978-981-99-4165-0. (doi:10.1007/978-981-99-4165-0_17) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:102447)
PDF
Publisher pdf
Language: English Restricted to Repository staff only |
|
Contact us about this Publication
|
![]() |
Official URL: https://doi.org/10.1007/978-981-99-4165-0_17 |
Abstract
In cows, there is a drastic metabolic stress caused by violations of the rumen microbiome composition during lactation. The aim of the present study was to investigate the α-and β-diversity of the rumen microbiome of lactating cows using next-generation sequencing (NGS) and gene expression assessed by qPCR, as well as evaluation of the corrective properties of a probiotic strain introduced into the rumen. The results showed that the addition of the probiotic to the diet contributed to an effective increase in the fat content of milk during the early lactation period (P ≤ 0.05). The NGS-based analysis of the rumen microbiome of the studied cows using the 16S rRNA gene sequences showed that the Shannon and Chao1 indices of α-diversity of prokaryotic communities remained unchanged during various feeding phases, as well as due to the probiotic effects. In the early lactation period and under the probiotic’s impact, the number of representatives of the Clostridia_UCG-014 and Clostridiaceae families declined by 2.4 and 1.6 times, respectively (in Group 2 as compared to Group 1, P ≤ 0.05). Expression of the bacterial Ldh-L and ldhD genes was lower by 2.9 and 13.5 times, respectively (P ≤ 0.05), when adding the probiotic to the diet in the early lactation period.
Item Type: | Conference or workshop item (Paper) |
---|---|
DOI/Identification number: | 10.1007/978-981-99-4165-0_17 |
Uncontrolled keywords: | Rumen microbiome; Gene expression; Probiotic; NGS sequencing; Lactating cows |
Subjects: |
Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology S Agriculture > SF Animal culture |
Divisions: |
Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction Divisions > Division of Natural Sciences > Biosciences |
Signature Themes: | Food Systems, Natural Resources and Environment |
Depositing User: | Mike Romanov |
Date Deposited: | 15 Aug 2023 09:48 UTC |
Last Modified: | 28 May 2024 17:11 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/102447 (The current URI for this page, for reference purposes) |
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):