Eastwood, Tara, Baker, Karen, Streather, Bree, Allen, Nyasha, Wang, Lin, Botchway, Stan W, Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P. and others. (2023) High yield vesicle packaged recombinant protein production from E. coli. Cell Reports Methods, 3 (2). Article Number 100396. ISSN 2667-2375. (doi:doi: 10.1016/j.crmeth.2023.100396) (KAR id:99506)
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Language: English DOI for this version: 10.22024/UniKent/01.02.99506.3357458 
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| Official URL: https://doi.org/doi: 10.1016/j.crmeth.2023.100396 |
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| Resource title: | High-yield vesicle-packaged recombinant protein production from E. coli |
|---|---|
| Resource type: | Dataset |
| DOI: | 10.22024/UniKent/01.01.416 |
| KDR/KAR URL: | https://data.kent.ac.uk/416/ |
| External URL: | https://doi.org/10.22024/UniKent/01.01.416 |
| Relationship: |
Abstract
We describe an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli. These recombinant vesicles compartmentalise proteins within a micro-environment that allows production of otherwise challenging insoluble, toxic, or disulphide-bond containing proteins from bacteria. The release of the inducible vesicle packaged soluble protein supports isolation from the culture within an environment allowing long term storage of active protein. This technology results in high-yields of vesicle packaged soluble functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | doi: 10.1016/j.crmeth.2023.100396 |
| Projects: | 22810 |
| Additional information: | For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. |
| Uncontrolled keywords: | Recombinant protein, Disulfide bond, Extracellular Vesicles |
| Subjects: |
Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH581.2 Cell Biology Q Science > QR Microbiology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Funders: | Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
| Depositing User: | Daniel Mulvihill |
| Date Deposited: | 13 Jan 2023 12:12 UTC |
| Last Modified: | 18 Jul 2023 14:50 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/99506 (The current URI for this page, for reference purposes) |
| Eastwood, Tara: |  https://orcid.org/0000-0003-2587-0574 |
| Baker, Karen: | https://orcid.org/0000-0001-7628-1978 |
| Streather, Bree: | https://orcid.org/0000-0003-0190-8558 |
| Allen, Nyasha: | https://orcid.org/0000-0002-9971-6118 |
| Wang, Lin: | https://orcid.org/0000-0001-9141-5217 |
| Botchway, Stan W: | https://orcid.org/0000-0002-3268-9303 |
| Brown, Ian R.: | https://orcid.org/0000-0002-6989-4474 |
| Hiscock, Jennifer R.: | https://orcid.org/0000-0002-1406-8802 |
| Lennon, Christopher: | https://orcid.org/0000-0002-2437-1485 |
| Mulvihill, Daniel P.: | https://orcid.org/0000-0003-2502-5274 |
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