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High yield vesicle packaged recombinant protein production from E. coli.

Eastwood, Tara, Baker, Karen, Streather, Bree, Allen, Nyasha, Wang, Lin, Botchway, Stan W, Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P. and others. (2023) High yield vesicle packaged recombinant protein production from E. coli. Cell Reports Methods, 3 (2). Article Number 100396. ISSN 2667-2375. (doi:doi: 10.1016/j.crmeth.2023.100396) (KAR id:99506)

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DOI for this version: 10.22024/UniKent/01.02.99506.3357458

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Official URL:
https://doi.org/doi: 10.1016/j.crmeth.2023.100396

Abstract

We describe an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli. These recombinant vesicles compartmentalise proteins within a micro-environment that allows production of otherwise challenging insoluble, toxic, or disulphide-bond containing proteins from bacteria. The release of the inducible vesicle packaged soluble protein supports isolation from the culture within an environment allowing long term storage of active protein. This technology results in high-yields of vesicle packaged soluble functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.

Item Type: Article
DOI/Identification number: doi: 10.1016/j.crmeth.2023.100396
Projects: 22810
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords: Recombinant protein, Disulfide bond, Extracellular Vesicles
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Depositing User: Daniel Mulvihill
Date Deposited: 13 Jan 2023 12:12 UTC
Last Modified: 20 Nov 2023 15:38 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/99506 (The current URI for this page, for reference purposes)
Eastwood, Tara: https://orcid.org/0000-0003-2587-0574
Baker, Karen: https://orcid.org/0000-0001-7628-1978
Streather, Bree: https://orcid.org/0000-0003-0190-8558
Allen, Nyasha: https://orcid.org/0000-0002-9971-6118
Wang, Lin: https://orcid.org/0000-0001-9141-5217
Botchway, Stan W: https://orcid.org/0000-0002-3268-9303
Brown, Ian R.: https://orcid.org/0000-0002-6989-4474
Hiscock, Jennifer R.: https://orcid.org/0000-0002-1406-8802
Lennon, Christopher: https://orcid.org/0000-0002-2437-1485
Mulvihill, Daniel P.: https://orcid.org/0000-0003-2502-5274
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