Eastwood, Tara, Baker, Karen, Streather, Bree, Allen, Nyasha, Wang, Lin, Botchway, Stan W, Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P. and others. (2023) High yield vesicle packaged recombinant protein production from E. coli. Cell Reports Methods, . ISSN 2667-2375. (doi:doi: 10.1016/j.crmeth.2023.100396) (KAR id:99506)
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Official URL: https://doi.org/doi: 10.1016/j.crmeth.2023.100396 |
Resource title: | High-yield vesicle-packaged recombinant protein production from E. coli |
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Resource type: | Dataset |
DOI: | 10.22024/UniKent/01.01.416 |
KDR/KAR URL: | https://data.kent.ac.uk/416/ |
External URL: | https://doi.org/10.22024/UniKent/01.01.416 |
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Abstract
We describe an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli. These recombinant vesicles compartmentalise proteins within a micro-environment that allows production of otherwise challenging insoluble, toxic, or disulphide-bond containing proteins from bacteria. The release of the inducible vesicle packaged soluble protein supports isolation from the culture within an environment allowing long term storage of active protein. This technology results in high-yields of vesicle packaged soluble functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
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