Skip to main content
Kent Academic Repository

High yield vesicle packaged recombinant protein production from E. coli.

Eastwood, Tara, Baker, Karen, Streather, Bree, Allen, Nyasha, Wang, Lin, Botchway, Stan W, Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P. and others. (2023) High yield vesicle packaged recombinant protein production from E. coli. Cell Reports Methods, 3 (2). Article Number 100396. ISSN 2667-2375. (doi:doi: 10.1016/j.crmeth.2023.100396) (KAR id:99506)

PDF (Published pdf including supplemental information) Publisher pdf
Language: English


Download this file
(PDF/7MB)
[thumbnail of Published pdf including supplemental information]
Preview
Request a format suitable for use with assistive technology e.g. a screenreader
PDF (Complete article, including supplemental information) Author's Accepted Manuscript
Language: English

DOI for this version: 10.22024/UniKent/01.02.99506.3357458

Download this file
(PDF/6MB)
[thumbnail of Complete article, including supplemental information]
Preview
Request a format suitable for use with assistive technology e.g. a screenreader
Official URL:
https://doi.org/doi: 10.1016/j.crmeth.2023.100396

Abstract

We describe an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli. These recombinant vesicles compartmentalise proteins within a micro-environment that allows production of otherwise challenging insoluble, toxic, or disulphide-bond containing proteins from bacteria. The release of the inducible vesicle packaged soluble protein supports isolation from the culture within an environment allowing long term storage of active protein. This technology results in high-yields of vesicle packaged soluble functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.

Item Type: Article
DOI/Identification number: doi: 10.1016/j.crmeth.2023.100396
Projects: 22810
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords: Recombinant protein, Disulfide bond, Extracellular Vesicles
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Depositing User: Daniel Mulvihill
Date Deposited: 13 Jan 2023 12:12 UTC
Last Modified: 20 Nov 2023 15:38 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/99506 (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.