Eastwood, Tara, Baker, Karen, Streather, Bree, Allen, Nyasha, Wang, Lin, Botchway, Stan W, Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P. and others. (2023) High yield vesicle packaged recombinant protein production from E. coli. Cell Reports Methods, 3 (2). Article Number 100396. ISSN 2667-2375. (doi:doi: 10.1016/j.crmeth.2023.100396) (KAR id:99506)
|
PDF (Published pdf including supplemental information)
Publisher pdf
Language: English 
This work is licensed under a Creative Commons Attribution 4.0 International License.
|
|
|
Download this file (PDF/7MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
|
PDF (Complete article, including supplemental information)
Author's Accepted Manuscript
Language: English DOI for this version: 10.22024/UniKent/01.02.99506.3357458 
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
|
|
|
Download this file (PDF/6MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
| Official URL: https://doi.org/doi: 10.1016/j.crmeth.2023.100396 |
|
| Resource title: | High-yield vesicle-packaged recombinant protein production from E. coli |
|---|---|
| Resource type: | Dataset |
| DOI: | 10.22024/UniKent/01.01.416 |
| KDR/KAR URL: | https://data.kent.ac.uk/416/ |
| External URL: | https://doi.org/10.22024/UniKent/01.01.416 |
Abstract
We describe an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli. These recombinant vesicles compartmentalise proteins within a micro-environment that allows production of otherwise challenging insoluble, toxic, or disulphide-bond containing proteins from bacteria. The release of the inducible vesicle packaged soluble protein supports isolation from the culture within an environment allowing long term storage of active protein. This technology results in high-yields of vesicle packaged soluble functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | doi: 10.1016/j.crmeth.2023.100396 |
| Projects: | 22810 |
| Additional information: | For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising. |
| Uncontrolled keywords: | Recombinant protein, Disulfide bond, Extracellular Vesicles |
| Subjects: |
Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH581.2 Cell Biology Q Science > QR Microbiology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Funders: | Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
| Depositing User: | Daniel Mulvihill |
| Date Deposited: | 13 Jan 2023 12:12 UTC |
| Last Modified: | 05 Nov 2024 13:05 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/99506 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Eastwood, Tara.
| Creator's ORCID: |
 https://orcid.org/0000-0003-2587-0574
|
|---|---|
| CReDIT Contributor Roles: |
Baker, Karen.
| Creator's ORCID: |
https://orcid.org/0000-0001-7628-1978
|
|---|---|
| CReDIT Contributor Roles: |
Streather, Bree.
| Creator's ORCID: |
https://orcid.org/0000-0003-0190-8558
|
|---|---|
| CReDIT Contributor Roles: |
Brown, Ian R..
| Creator's ORCID: |
https://orcid.org/0000-0002-6989-4474
|
|---|---|
| CReDIT Contributor Roles: |
Hiscock, Jennifer R..
| Creator's ORCID: |
https://orcid.org/0000-0002-1406-8802
|
|---|---|
| CReDIT Contributor Roles: |
Mulvihill, Daniel P..
| Creator's ORCID: |
https://orcid.org/0000-0003-2502-5274
|
|---|---|
| CReDIT Contributor Roles: |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric
