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Application and comparison of lyophilisation protocols to enhance stable long-term storage of filovirus pseudotypes for use in antibody neutralisation tests

Mayora-Neto, Martin, Wright, Edward, Temperton, Nigel J., Soema, Peter, ten Have, Rimko, Ploemen, Ivo, Scott, Simon D. (2022) Application and comparison of lyophilisation protocols to enhance stable long-term storage of filovirus pseudotypes for use in antibody neutralisation tests. Journal of Applied Microbiology, . E-ISSN 1365-2672. (doi:10.1093/jambio/lxac067) (KAR id:97831)

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Official URL:
https://doi.org/10.1093/jambio/lxac067

Abstract

Filoviruses encompass highly pathogenic viruses placing significant public health burden on countries affected. Efforts for improved diagnostics and surveillance are needed. The requirement for high-containment can be circumvented by using pseudotype viruses (PV), which can be handled safely, in tropism, drug screening, vaccine evaluation and serosurveillance studies. We assessed stability and functionality after long-term storage of lyophilised filovirus pseudotypes for use in neutralisation assays

Item Type: Article
DOI/Identification number: 10.1093/jambio/lxac067
Projects: 215 22415
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Funders: Medway School of Pharmacy (https://ror.org/00fa9v295)
University of Sussex (https://ror.org/00ayhx656)
Depositing User: Simon Scott
Date Deposited: 17 Nov 2022 14:28 UTC
Last Modified: 26 Jan 2023 11:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/97831 (The current URI for this page, for reference purposes)
Mayora-Neto, Martin: https://orcid.org/0000-0002-5331-5554
Temperton, Nigel J.: https://orcid.org/0000-0002-7978-3815
Scott, Simon D.: https://orcid.org/0000-0002-8290-0461
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