Exploration of Factors Affecting Recombinant Protein Secretion in Saccharomyces Cerevisiae

Production of recombinant proteins is a vital industry to overcome limited and costly natural supplies. The huge industry of recombinant protein production and purification relies on multiple cell types and genetic manipulation tools. Saccharomyces cerevisiae is a promising organism to produce recombinant protein due to the ability to secrete recombinant protein into the extracellular media. Secretion into the extracellular media allows for simpler purification of proteins, but is hampered by low yields. In this study we approach recombinant protein production in Saccharomyces cerevisiae from different angles to study the impact upon protein yields and secretion capability. Initially looking at the effects of genetic deletions, then tuning of the endoplasmic reticulum and finally consideration of different strains and growth conditions. When this data is taken together, it shows the necessity of tuning individual systems to enable the best conditions, as different cell types react differently to conditions which have been optimised for another. The variation in cell types shows that single breakthroughs which can be applied to all systems cannot be expected, and studies must consider effects among a broad selection of strains to enable universal understanding of its affects upon protein production and secretion as a whole.