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Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds

Nikitkina, Elena V., Dementieva, Natalia V., Shcherbakov, Yuri S., Atroshchenko, Mikhail M., Kudinov, Andrei A., Samoylov, Oleg I., Pozovnikova, Marina V., Dysin, Artem P., Krutikova, A A, Musidray, Artem A., and others. (2022) Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds. Animal Bioscience, 35 (12). pp. 1827-1838. ISSN 2765-0189. E-ISSN 2765-0235. (doi:10.5713/ab.21.0504) (KAR id:96956)

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Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds.

Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom™ Equine Genotyping Array.

Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME8, OR2AP1 and OR6C4. Potential implications of effects of these genes on sperm motility are herein discussed.

Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

Item Type: Article
DOI/Identification number: 10.5713/ab.21.0504
Uncontrolled keywords: candidate genes; cryopreservation; Equus caballus; SNPs; sperm; stallion
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH75 Conservation (Biology)
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction
Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 16 Sep 2022 05:13 UTC
Last Modified: 25 Oct 2022 05:19 UTC
Resource URI: (The current URI for this page, for reference purposes)
Nikitkina, Elena V.:
Dementieva, Natalia V.:
Shcherbakov, Yuri S.:
Atroshchenko, Mikhail M.:
Kudinov, Andrei A.:
Samoylov, Oleg I.:
Pozovnikova, Marina V.:
Dysin, Artem P.:
Krutikova, A A:
Musidray, Artem A.:
Mitrofanova, Olga V.:
Plemyashov, Kirill V.:
Griffin, Darren K.:
Romanov, Michael N.:
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