Hothersall, Joanne, Lai, Sandie, Zhang, Nan, Godfrey, Rita E., Ruanto, Patcharawarin, Bischoff, Sarah, Robinson, Colin, Overton, Tim W., Busby, Stephen J. W., Browning, Douglas F. and others. (2022) Inexpensive protein overexpression driven by the NarL transcription activator protein. Biotechnology and Bioengineering, 119 (6). pp. 1614-1623. ISSN 0006-3592. (doi:10.1002/bit.28071) (KAR id:96806)
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| Official URL: https://doi.org/10.1002/bit.28071 |
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Abstract
Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. However, these can be expensive and, sometimes, chemically unstable. To simplify this and to cut the cost of RPP, we have developed vectors controlled by the Escherichia coli nitrate-responsive NarL transcription activator protein, which use nitrate, a cheap, stable, and abundant inorganic ion, to induce high-level controlled RPP. We show that target proteins, such as green fluorescent protein, human growth hormone, and single-chain variable region antibody fragments can be expressed to high levels using our promoter systems. As nitrate levels are high in many commercial fertilizers, we demonstrate that controlled RPP can be achieved using readily available and inexpensive garden products.
| Item Type: | Article |
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| DOI/Identification number: | 10.1002/bit.28071 |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Funders: |
Innovate UK (https://ror.org/05ar5fy68)
Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
| Depositing User: | Colin Robinson |
| Date Deposited: | 08 Sep 2022 01:40 UTC |
| Last Modified: | 05 Nov 2024 13:01 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/96806 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Robinson, Colin.
| Creator's ORCID: |
 https://orcid.org/0000-0002-1739-032X
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