Evaluation of Methylotrophic Yeast Ogataea thermomethanolica TBRC 656 as a Heterologous Host for Production of an Animal Vaccine Candidate

Liwnaree, Benjamas, Muensaen, Katanchalee, Narkpuk, Jaraspim, Promdonkoy, Peerada, Kocharin, Kanokarn, Peswani, Amber R., Robinson, Colin, Mikaliunaite, Lina, Roongsawang, Niran, Tanapongpipat, Sutipa, and others. (2022) Evaluation of Methylotrophic Yeast Ogataea thermomethanolica TBRC 656 as a Heterologous Host for Production of an Animal Vaccine Candidate. Molecular Biotechnology, . ISSN 1073-6085. (doi:10.1007/s12033-022-00508-x) (KAR id:96805)

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Official URL: https://doi.org/10.1007/s12033-022-00508-x

Abstract

Multiple yeast strains have been developed into versatile heterologous protein expression platforms. Earlier works showed that Ogataea thermomethanolica TBRC 656 (OT), a thermotolerant methylotrophic yeast, can efficiently produce several industrial enzymes. In this work, we demonstrated the potential of this platform for biopharmaceutical manufacturing. Using a swine vaccine candidate as a model, we showed that OT can be optimized to express and secrete the antigen based on porcine circovirus type 2d capsid protein at a respectable yield. Crucial steps for yield improvement include codon optimization and reduction of OT protease activities. The antigen produced in this system could be purified efficiently and induce robust antibody response in test animals. Improvements in this platform, especially more efficient secretion and reduced extracellular proteases, would extend its potential as a competitive platform for biopharmaceutical industries.

Item Type:	Article
DOI/Identification number:	10.1007/s12033-022-00508-x
Uncontrolled keywords:	Porcine circovirus type 2, Veterinary vaccine, Ogataea thermomethanolica, Heterologous expression, Methylotrophic yeast
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Funders:	UK Research and Innovation (https://ror.org/001aqnf71)
Depositing User:	Colin Robinson
Date Deposited:	08 Sep 2022 01:33 UTC
Last Modified:	04 Mar 2024 18:27 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/96805 (The current URI for this page, for reference purposes)

University of Kent Author Information

Robinson, Colin.

Creator's ORCID:	https://orcid.org/0000-0002-1739-032X
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