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3D chromatin remodelling in the germ line modulates genome evolutionary plasticity

Alvarez-Gonzalez, Lucia, Burden, Frances, Doddamani, Dadakhalandar, Malinverni, Roberto, Leach, Emma, Marín-García, Cristina, Marin-Gual, Laia, Gubern, Albert, Vara, Covadonga, Paytuví-Gallart, Andreu, and others. (2022) 3D chromatin remodelling in the germ line modulates genome evolutionary plasticity. Nature Communications, 13 (2608). ISSN 2041-1723. (doi:10.1038/s41467-022-30296-6) (KAR id:95043)

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Abstract

Chromosome folding has profound impacts on gene regulation, whose evolutionary consequences are far from being understood. Here we explore the relationship between 3D chromatin remodelling in mouse germ cells and evolutionary changes in genome structure. Using a comprehensive integrative computational analysis, we (i) reconstruct seven ancestral rodent genomes analysing whole-genome sequences of 14 species representatives of the major phylogroups, (ii) detect lineage-specific chromosome rearrangements and (iii) identify the dynamics of the structural and epigenetic properties of evolutionary breakpoint regions(EBRs) throughout mouse spermatogenesis. Our results show that EBRs are devoid of programmed meiotic DNA double-strand breaks (DSBs) and meiotic cohesins in primary spermatocytes, but are associated in post-meiotic cells with sites of DNA damage and functional long-range interaction regions that recapitulate ancestral chromosomal configurations. Overall, we propose a model that integrates evolutionary genome reshuffling with DNA damage response mechanisms and the dynamic spatial genome organisation of germ cells

Item Type: Article
DOI/Identification number: 10.1038/s41467-022-30296-6
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH324.2 Computational biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Marta Farre Belmonte
Date Deposited: 16 May 2022 10:35 UTC
Last Modified: 17 May 2022 11:55 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/95043 (The current URI for this page, for reference purposes)
Ellis, Peter J.I.: https://orcid.org/0000-0001-9709-7934
Farré, Marta: https://orcid.org/0000-0001-9170-5767
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