Hughes, Colin (1978) Studies on antibiotic-resistant enterobacteria isolated from polluted river water. Doctor of Philosophy (PhD) thesis, University of Kent. (doi:10.22024/UniKent/01.02.94429) (KAR id:94429)
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| Official URL: https://doi.org/10.22024/UniKent/01.02.94429 |
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Abstract
Two methods were devised to simplify the examination of antibiotic-resistant enterobacteria from natural sources.
The efficient isolation of enterobacteria from polluted river water is allowed by supplementing bile salts medium with a critically small amount of nalidixic acid. This medium allows direct counting of coliforms by eliminating all other lactose fermenters.
A further procedure is described for demonstrating plasmid DNA and it's molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each plasmid species, nicked during preparation of lysates, forms only a single band, thus simplifying the examination of plasmids from naturally occurring strains.
These methods were used to investigate standard colicinogenic strains and wild-type enterobacteria isolated from polluted river water. The effect of plasmid-carriage on the survival of enterobacteria was examined in river water and also in serum.
| Item Type: | Thesis (Doctor of Philosophy (PhD)) |
|---|---|
| DOI/Identification number: | 10.22024/UniKent/01.02.94429 |
| Additional information: | This thesis has been digitised by EThOS, the British Library digitisation service, for purposes of preservation and dissemination. It was uploaded to KAR on 25 April 2022 in order to hold its content and record within University of Kent systems. It is available Open Access using a Creative Commons Attribution, Non-commercial, No Derivatives (https://creativecommons.org/licenses/by-nc-nd/4.0/) licence so that the thesis and its author, can benefit from opportunities for increased readership and citation. This was done in line with University of Kent policies (https://www.kent.ac.uk/is/strategy/docs/Kent%20Open%20Access%20policy.pdf). If you feel that your rights are compromised by open access to this thesis, or if you would like more information about its availability, please contact us at ResearchSupport@kent.ac.uk and we will seriously consider your claim under the terms of our Take-Down Policy (https://www.kent.ac.uk/is/regulations/library/kar-take-down-policy.html). |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| SWORD Depositor: | SWORD Copy |
| Depositing User: | SWORD Copy |
| Date Deposited: | 14 Jul 2023 15:06 UTC |
| Last Modified: | 05 Nov 2024 12:59 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/94429 (The current URI for this page, for reference purposes) |
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