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Development of pGEMINI, a Plant Gateway Destination Vector Allowing the Simultaneous Integration of Two cDNA via a Single LR-Clonase Reaction

Exposito-Rodriguez, Marino, Laissue, Philippe P., López-Calcagno, Patricia E., Mullineaux, Philip M., Raines, Christine A., Simkin, Andrew J. (2017) Development of pGEMINI, a Plant Gateway Destination Vector Allowing the Simultaneous Integration of Two cDNA via a Single LR-Clonase Reaction. Plants, 6 (4). p. 55. ISSN 2223-7747. E-ISSN 1741-3117. (doi:10.3390/plants6040055) (KAR id:93903)

Abstract

Gateway technology has been used to facilitate the generation of a large number of constructs for the modification of plants for research purposes. However, many of the currently available vectors only allow the integration of a single cDNA of interest into an expression clone. The ability to over-express multiple genes in combination is essential for the study of plant development where several transcripts have a role to play in one or more metabolic processes. The tools to carry out such studies are limited, and in many cases rely on the incorporation of cDNA into expression systems via conventional cloning, which can be both time consuming and laborious. To our knowledge, this study reports on the first development of a vector allowing the simultaneous integration of two independent cDNAs via a single LR-clonase reaction. This vector “pGEMINI” represents a powerful molecular tool offering the ability to study the role of multi-cDNA constructs on plant development, and opens up the process of gene stacking and the study of gene combinations through transient or stable transformation procedures.

Item Type: Article
DOI/Identification number: 10.3390/plants6040055
Uncontrolled keywords: gateway; gene stacking; LR clonase; pGEMINI; fluorescence
Subjects: Q Science > QH Natural history
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Andrew Simkin
Date Deposited: 05 May 2022 17:24 UTC
Last Modified: 05 Nov 2024 12:59 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93903 (The current URI for this page, for reference purposes)

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