Simkin, Andrew J., Guirimand, Grégory, Papon, Nicolas, Courdavault, Vincent, Thabet, Insaf, Ginis, Olivia, Bouzid, Sadok, Giglioli-Guivarc’h, Nathalie, Clastre, Marc (2011) Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta. Planta, 234 (5). pp. 903-914. ISSN 0032-0935. E-ISSN 1432-2048. (doi:10.1007/s00425-011-1444-6) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:93882)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: https://doi.org/10.1007/s00425-011-1444-6 |
Abstract
In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final steps of the plant MVA pathway are localised to the peroxisome.
Item Type: | Article |
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DOI/Identification number: | 10.1007/s00425-011-1444-6 |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Andrew Simkin |
Date Deposited: | 05 May 2022 18:46 UTC |
Last Modified: | 06 May 2022 09:20 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/93882 (The current URI for this page, for reference purposes) |
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