Simkin, Andrew J., Lopez-Calcagno, Patricia E., Davey, Philip A., Headland, Lauren R., Lawson, Tracy, Timm, Stefan, Bauwe, Hermann, Raines, Christine A. (2017) Simultaneous stimulation of sedoheptulose 1,7-bisphosphatase, fructose 1,6-bisphophate aldolase and the photorespiratory glycine decarboxylase-H protein increases CO2assimilation, vegetative biomass and seed yield in Arabidopsis. Plant Biotechnology Journal, 15 (7). pp. 805-816. ISSN 1467-7644. E-ISSN 1467-7652. (doi:10.1111/pbi.12676) (KAR id:93875)
PDF
Publisher pdf
Language: English
This work is licensed under a Creative Commons Attribution 4.0 International License.
|
|
Download this file (PDF/1MB) |
Preview |
Request a format suitable for use with assistive technology e.g. a screenreader | |
Official URL: https://doi.org/10.1111/pbi.12676 |
Abstract
In this article, we have altered the levels of three different enzymes involved in the Calvin–Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7-bisphosphatase (SBPase), fructose 1,6-bisphophate aldolase (FBPA) and the glycine decarboxylase-H protein (GDC-H) gene identified as targets to improve photosynthesis based on previous studies. Here, we show that increasing the levels of the three corresponding proteins, either independently or in combination, significantly increases the quantum efficiency of PSII. Furthermore, photosynthetic measurements demonstrated an increase in the maximum efficiency of CO2 fixation in lines over-expressing SBPase and FBPA. Moreover, the co-expression of GDC-H with SBPase and FBPA resulted in a cumulative positive impact on leaf area and biomass. Finally, further analysis of transgenic lines revealed a cumulative increase of seed yield in SFH lines grown in high light. These results demonstrate the potential of multigene stacking for improving the productivity of food and energy crops.
Item Type: | Article |
---|---|
DOI/Identification number: | 10.1111/pbi.12676 |
Uncontrolled keywords: | chlorophyll fluorescence imaging; FBP aldolase; glycine decarboxylase-H protein; photosynthesis; SBPase; transgenic |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Andrew Simkin |
Date Deposited: | 05 May 2022 18:06 UTC |
Last Modified: | 06 May 2022 08:41 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/93875 (The current URI for this page, for reference purposes) |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):