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CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes

Douglas, Charlotte, Maciulyte, Valdone, Zohren, Jasmin, Snell, Daniel M., Mahadevaiah, Shantha K., Ojarikre, Obah A., Ellis, Peter J.I., Turner, James M.A. (2021) CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes. Nature Communications, 12 . Article Number 6926. ISSN 2041-1723. (doi:10.1038/s41467-021-27227-2) (KAR id:93108)

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https://doi.org/10.1038/s41467-021-27227-2

Abstract

Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male-or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture.

Item Type: Article
DOI/Identification number: 10.1038/s41467-021-27227-2
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QL Zoology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Peter Ellis
Date Deposited: 07 Feb 2022 18:17 UTC
Last Modified: 08 Feb 2022 10:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93108 (The current URI for this page, for reference purposes)
Ellis, Peter J.I.: https://orcid.org/0000-0001-9709-7934
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