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Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study

Silvestri, Giuseppe, Canedo-Ribeiro, Carla, Serrano-Albal, María, Labrecque, Remi, Blondin, Patrick, Larmer, Steven G., Marras, Gabriele, Tutt, Desmond A.R., Handyside, Alan H., Farré, Marta, and others. (2021) Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study. Cells, 10 (9). Article Number 2284. ISSN 2073-4409. (doi:10.3390/cells10092284) (KAR id:90024)

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Official URL
https://doi.org/10.3390/cells10092284

Abstract

Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.

Item Type: Article
DOI/Identification number: 10.3390/cells10092284
Uncontrolled keywords: cattle; breeding; single nucleotide polymorphism (SNP); mosaicism
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Giuseppe Silvestri
Date Deposited: 03 Sep 2021 15:21 UTC
Last Modified: 06 Sep 2021 13:39 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/90024 (The current URI for this page, for reference purposes)
Silvestri, Giuseppe: https://orcid.org/0000-0002-5496-2470
Farré, Marta: https://orcid.org/0000-0001-9170-5767
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
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