Skip to main content
Kent Academic Repository

Determination of microbiocoenosis in the intestine of the Hisex Brown hens in ontogenesis using T-RFLP method

Romanov, Michael N, Laptev, Georgi Yu., Nikonov, I N, Ilyina, Larisa A., Novikova, Natalia I., Barkova, Olga Y., Griffin, Darren K., Kochish, Ivan I., Smolensky, Vladimir I., Panin, A N, and others. (2017) Determination of microbiocoenosis in the intestine of the Hisex Brown hens in ontogenesis using T-RFLP method. In: Insights in Nutrition and Metabolism. Proceedings of 15th World Congress on Advances in Nutrition Food Science & Technology. 1 (3). p. 39. Allied Academies – UK, London, UK (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:89545)

PDF Publisher pdf
Language: English

Restricted to Repository staff only
Contact us about this Publication
[thumbnail of Romanov&al2017(Insights Nutr Metab - T-RFLP).pdf]
Official URL:


Microbiocoenosis in the gastrointestinal tract, especially in the ceca, play an important part in life processes of poultry. Identification of the structure and taxonomic composition of microorganisms in the cecum using molecular genetic methods serves as a crucial approach in understanding how a cecal microbiota interplays with the chicken organism during ontogenesis. For this purpose, we studied an intestinal bacterial community composition in the ceca of the Hisex Brown laying hens at age of 40, 155 and 315 days using T-RFLP and RTPCR. In the chickens studied, development of the cecal microbial communities, changes in their content, and appearance of new microorganisms occurred in the ontogeny. A broader spectrum of bacteria was found in 40- and 155-day-old birds (221 ± 11 and 258 ± 9 phylotypes, respectively) as compared with 315-day-old laying hens (178 ± 8 phylotypes). Also, 315-day-old birds showed the least content of unidentified phylotypes. In the ceca of adult hens, there was a change in the dominant microbial taxonomic groups including a higher proportion of acidutilising bacteria of the class Negativicutes and cellulolytic bacteria of the class Clostridia, with a lower content of the classes Bifidobacteriales and Bacillales. Lactobacteria (order Lactobacillales) showed a greater content in 315-day-old laying hens (33.15 ± 1.05%) as compared with 40- and 155-day-old birds (5.13 ± 0.23% and 24.58 ± 0.86%, respectively). The variety and number of bacteria in the ceca conventionally attributed to various pathogens of poultry diseases, including the genera Enterobacter, Pantoea, Listeria, Acinetobacter and Mycoplasma, families Campylobacteraceae and Pasteurellaceae, and phylum Fusobacteria, increased with the age of birds. Thus, during molecular genetic studies, the species composition and dynamics of the microbiocoenosis in the cecum of the Hisex Brown laying hens was determined as related to their ontogeny.

Item Type: Conference or workshop item (Paper)
Projects: Development of State-of-the-Art Biotechnologies to Assess Gene Expression in Relation to Performance and Disease Resistance in Poultry Industry
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Funders: Organisations -1 not found.
Depositing User: Mike Romanov
Date Deposited: 31 Jul 2021 13:20 UTC
Last Modified: 19 Jun 2023 13:55 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.