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Comparative mapping of the macrochromosomes of eight avian species provides further insight into their phylogenetic relationships and avian karyotype evolution

Kiazim, Lucas G., O’Connor, Rebecca E., Larkin, Denis M., Romanov, Michael N., Narushin, Valeriy G., Brazhnik, Evgeni A., Griffin, Darren K. (2021) Comparative mapping of the macrochromosomes of eight avian species provides further insight into their phylogenetic relationships and avian karyotype evolution. Cells, 10 (2). Article Number 362. E-ISSN 2073-4409. (doi:10.3390/cells10020362) (KAR id:89168)

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Official URL
https://doi.org/10.3390/cells10020362

Abstract

Avian genomes typically consist of ~10 pairs of macro- and ~30 pairs of microchromosomes. While inter-chromosomally, a pattern emerges of very little change (with notable exceptions) throughout evolution, intrachromosomal changes remain relatively poorly studied. To rectify this, here we use a pan-avian universally hybridising set of 74 chicken bacterial artificial chromosome (BAC) probes on the macrochromosomes of eight bird species: common blackbird, Atlantic canary, Eurasian woodcock, helmeted guinea fowl, houbara bustard, mallard duck, and rock dove. A combination of molecular cytogenetic, bioinformatics, and mathematical analyses allowed the building of comparative cytogenetic maps, reconstruction of a putative Neognathae ancestor, and assessment of chromosome rearrangement patterns and phylogenetic relationships in the studied neognath lineages. We observe that, as with our previous studies, chicken appears to have the karyotype most similar to the ancestor; however, previous reports of an increased rate of intrachromosomal change in Passeriformes (songbirds) appear not to be the case in our dataset. The use of this universally hybridizing probe set is applicable not only for the re-tracing of avian karyotype evolution but, potentially, for reconstructing genome assemblies.

Item Type: Article
DOI/Identification number: 10.3390/cells10020362
Projects: [UNSPECIFIED] BBSRC-BB/P020054/1 awarded to D.K.G.
[UNSPECIFIED] BB/K008226/1 awarded to D.M.L.
Uncontrolled keywords: avian species; macrochromosome; comparative cytogenetic maps; BACs; chromosome rearrangements; phylogenomics
Subjects: Q Science > QH Natural history > QH324.2 Computational biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QL Zoology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 12 Jul 2021 08:17 UTC
Last Modified: 30 Jul 2021 17:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/89168 (The current URI for this page, for reference purposes)
O’Connor, Rebecca E.: https://orcid.org/0000-0002-4270-970X
Larkin, Denis M.: https://orcid.org/0000-0001-7859-6201
Romanov, Michael N.: https://orcid.org/0000-0003-3584-4644
Narushin, Valeriy G.: https://orcid.org/0000-0001-6799-6605
Brazhnik, Evgeni A.: https://orcid.org/0000-0003-2178-9330
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
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