Kiazim, Lucas G., O’Connor, Rebecca E., Larkin, Denis M., Romanov, Michael N., Narushin, Valeriy G., Brazhnik, Evgeni A., Griffin, Darren K. (2021) Comparative mapping of the macrochromosomes of eight avian species provides further insight into their phylogenetic relationships and avian karyotype evolution. Cells, 10 (2). Article Number 362. ISSN 2073-4409. E-ISSN 2073-4409. (doi:10.3390/cells10020362) (KAR id:89168)
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Official URL: https://doi.org/10.3390/cells10020362 |
Abstract
Avian genomes typically consist of ~10 pairs of macro- and ~30 pairs of microchromosomes. While inter-chromosomally, a pattern emerges of very little change (with notable exceptions) throughout evolution, intrachromosomal changes remain relatively poorly studied. To rectify this, here we use a pan-avian universally hybridising set of 74 chicken bacterial artificial chromosome (BAC) probes on the macrochromosomes of eight bird species: common blackbird, Atlantic canary, Eurasian woodcock, helmeted guinea fowl, houbara bustard, mallard duck, and rock dove. A combination of molecular cytogenetic, bioinformatics, and mathematical analyses allowed the building of comparative cytogenetic maps, reconstruction of a putative Neognathae ancestor, and assessment of chromosome rearrangement patterns and phylogenetic relationships in the studied neognath lineages. We observe that, as with our previous studies, chicken appears to have the karyotype most similar to the ancestor; however, previous reports of an increased rate of intrachromosomal change in Passeriformes (songbirds) appear not to be the case in our dataset. The use of this universally hybridizing probe set is applicable not only for the re-tracing of avian karyotype evolution but, potentially, for reconstructing genome assemblies.
Item Type: | Article |
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DOI/Identification number: | 10.3390/cells10020362 |
Projects: | BBSRC-BB/P020054/1 awarded to D.K.G., BB/K008226/1 awarded to D.M.L. |
Uncontrolled keywords: | avian species; macrochromosome; comparative cytogenetic maps; BACs; chromosome rearrangements; phylogenomics |
Subjects: |
Q Science > QH Natural history > QH324.2 Computational biology Q Science > QH Natural history > QH426 Genetics Q Science > QH Natural history > QH581.2 Cell Biology Q Science > QL Zoology |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Signature Themes: | Food Systems, Natural Resources and Environment |
Funders: | Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982) |
Depositing User: | Mike Romanov |
Date Deposited: | 12 Jul 2021 08:17 UTC |
Last Modified: | 04 Mar 2024 18:16 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/89168 (The current URI for this page, for reference purposes) |
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