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Transketolase catalysed upgrading of l-arabinose: the one-step stereoselective synthesis of l-gluco-heptulose

Subrizi, Fabiana, Cárdenas-Fernández, Max, Lye, Gary J., Ward, John M., Dalby, Paul A., Sheppard, Tom D., Hailes, Helen C. (2016) Transketolase catalysed upgrading of l-arabinose: the one-step stereoselective synthesis of l-gluco-heptulose. Green Chemistry, 18 (10). pp. 3158-3165. ISSN 1463-9262. E-ISSN 1463-9270. (doi:10.1039/c5gc02660a) (KAR id:88160)

Abstract

Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.

Item Type: Article
DOI/Identification number: 10.1039/c5gc02660a
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Max Cardenas Fernandez
Date Deposited: 14 May 2021 15:40 UTC
Last Modified: 05 Nov 2024 12:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/88160 (The current URI for this page, for reference purposes)

University of Kent Author Information

Cárdenas-Fernández, Max.

Creator's ORCID: https://orcid.org/0000-0003-1422-5369
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