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Effects of essential oils-based supplement and Salmonella infection on gene expression, blood parameters, cecal microbiome and egg production in laying hens

Laptev, Georgi Yu., Yildirim, Elena A., Ilina, Larisa A., Filippova, Valentina A., Kochish, Ivan I., Gorfunkel, Elena P., Dubrovin, Andrei V., Brazhnik, Evgeni A., Narushin, Valeriy G., Novikova, Natalia I., and others. (2021) Effects of essential oils-based supplement and Salmonella infection on gene expression, blood parameters, cecal microbiome and egg production in laying hens. Animals, 11 (2). Article Number 360. ISSN 2076-2615. (doi:10.3390/ani11020360) (KAR id:85944)

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Official URL
https://doi.org/10.3390/ani11020360

Abstract

Simple Summary

One of the main roles in poultry resistance to infections caused by Salmonella is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Salmonella Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio®. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined. Cecal microbiome composition and blood biochemical/immunological indices were also explored and egg production traits recorded. As a result, the SE challenge of laying hens and Intebio® administration had either a suppressive or activating effect on the expression level of the studied genes (e.g., IL6 and BPIFB3), the latter echoing mammalian/human tissue-specific expression. There were also effects of the pathogen challenge and phytobiotic intake on the cecal microbiome profiles and blood biochemical/immunological parameters, including those reflecting the activity of the birds’ immune systems (e.g., serum bactericidal activity, β-lysine content, and immunoglobulin levels). Significant differences between control and experimental subgroups in egg performance traits (i.e., egg weight/number/mass) were also found. The phytobiotic administration suggested a positive effect on the welfare and productivity of poultry.

Item Type: Article
DOI/Identification number: 10.3390/ani11020360
Projects: [UNSPECIFIED] Development of State-of-the-Art Biotechnologies to Assess Gene Expression in Relation to Performance and Disease Resistance in Poultry Industry
Additional information: Prof. Dr. Vladimir I. Smolensky died on 23 January 2021.
Uncontrolled keywords: essential oils-based phytobiotic; Salmonella enterica serovar Enteritidis; gene expression; cecum microbiome; blood biochemical/immunological parameters; laying hens
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 09 Feb 2021 15:28 UTC
Last Modified: 12 Jul 2021 11:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/85944 (The current URI for this page, for reference purposes)
Laptev, Georgi Yu.: https://orcid.org/0000-0002-8795-6659
Yildirim, Elena A.: https://orcid.org/0000-0002-5846-5105
Ilina, Larisa A.: https://orcid.org/0000-0003-2789-4844
Filippova, Valentina A.: https://orcid.org/0000-0001-8789-9837
Kochish, Ivan I.: https://orcid.org/0000-0002-8502-6052
Gorfunkel, Elena P.: https://orcid.org/0000-0002-6843-8733
Dubrovin, Andrei V.: https://orcid.org/0000-0001-8424-4114
Brazhnik, Evgeni A.: https://orcid.org/0000-0003-2178-9330
Narushin, Valeriy G.: https://orcid.org/0000-0001-6799-6605
Novikova, Natalia I.: https://orcid.org/0000-0002-9647-4184
Novikova, Oksana B.: https://orcid.org/0000-0003-0046-625X
Dunyashev, Timur P.: https://orcid.org/0000-0002-3918-0948
Smolensky, Vladimir I.: https://orcid.org/0000-0002-2124-0017
Surai, Peter F.: https://orcid.org/0000-0002-5012-8681
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
Romanov, Michael N.: https://orcid.org/0000-0003-3584-4644
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