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Effects of essential oils-based supplement and Salmonella infection on gene expression, blood parameters, cecal microbiome and egg production in laying hens

Laptev, Georgi Yu., Yildirim, Elena A., Ilina, Larisa A., Filippova, Valentina A., Kochish, Ivan I., Gorfunkel, Elena P., Dubrovin, Andrei V., Brazhnik, Evgeni A., Narushin, Valeriy G., Novikova, Natalia I., and others. (2021) Effects of essential oils-based supplement and Salmonella infection on gene expression, blood parameters, cecal microbiome and egg production in laying hens. Animals, 11 (2). Article Number 360. ISSN 2076-2615. E-ISSN 2076-2615. (doi:10.3390/ani11020360) (KAR id:85944)


Simple Summary

Salmonellosis is one of the most severe zoonotic diseases transmitted to humans through animal products (especially poultry meat and eggs). Essential oils (EOs)-based feed additives in poultry nutrition are a possible alternative replacement of antimicrobials to fight this infection. In the present study on laying hens, we tested a phytobiotic, Intebio®, and elucidated formation of immune response and changes in cecal microbiocenosis and biochemical/immunological variables in blood caused by Salmonella. Changes in differential gene expression were observed at both one and seven days post-inoculation in the hens’ intestines, revealing similarities with known mammalian/human tissue-specific expression. The results of this study suggest that the challenge of birds with Salmonella had a negative effect, while phytobiotic intake had a positive effect on the status of their gastrointestinal microbiome, their level of metabolism, and their performance.


One of the main roles in poultry resistance to infections caused by Salmonella is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Salmonella Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio®. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined. Cecal microbiome composition and blood biochemical/immunological indices were also explored and egg production traits recorded. As a result, the SE challenge of laying hens and Intebio® administration had either a suppressive or activating effect on the expression level of the studied genes (e.g., IL6 and BPIFB3), the latter echoing mammalian/human tissue-specific expression. There were also effects of the pathogen challenge and phytobiotic intake on the cecal microbiome profiles and blood biochemical/immunological parameters, including those reflecting the activity of the birds’ immune systems (e.g., serum bactericidal activity, β-lysine content, and immunoglobulin levels). Significant differences between control and experimental subgroups in egg performance traits (i.e., egg weight/number/mass) were also found. The phytobiotic administration suggested a positive effect on the welfare and productivity of poultry.

Item Type: Article
DOI/Identification number: 10.3390/ani11020360
Projects: Development of State-of-the-Art Biotechnologies to Assess Gene Expression in Relation to Performance and Disease Resistance in Poultry Industry
Additional information: Prof. Dr. Vladimir I. Smolensky died on 23 January 2021.
Uncontrolled keywords: essential oils-based phytobiotic; Salmonella enterica serovar Enteritidis; gene expression; cecum microbiome; blood biochemical/immunological parameters; laying hens
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 09 Feb 2021 15:28 UTC
Last Modified: 08 Sep 2023 13:42 UTC
Resource URI: (The current URI for this page, for reference purposes)

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