Zielke, Norman, Korzelius, Jerome, van Straaten, Monique, Bender, Katharina, Schuhknecht, Gregor F.P., Dutta, Devanjali, Xiang, Jinyi, Edgar, Bruce A. (2014) Fly-FUCCI: A Versatile Tool for Studying Cell Proliferation in Complex Tissues. Cell Reports, 7 (2). pp. 588-598. ISSN 2211-1247. (doi:10.1016/j.celrep.2014.03.020) (KAR id:81269)
PDF
Publisher pdf
Language: English
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
|
|
Download this file (PDF/4MB) |
![]() |
Request a format suitable for use with assistive technology e.g. a screenreader | |
Official URL: https://doi.org/10.1016/j.celrep.2014.03.020 |
Abstract
One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI relies on fluorochrome-tagged degrons from the Cyclin B and E2F1 proteins, which are degraded by the ubiquitin E3-ligases APC/C and CRL4Cdt2, during mitosis or the onset of S phase, respectively. These probes can track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and probably other tissues. To support a broad range of experimental applications, we have generated a toolkit of transgenic Drosophila lines that express the Fly-FUCCI probes under control of the UASt, UASp, QUAS, and ubiquitin promoters. The Fly-FUCCI system should be a valuable tool for visualizing cell-cycle activity during development, tissue homeostasis, and neoplastic growth.
Note: Graphical abstract on article
Item Type: | Article |
---|---|
DOI/Identification number: | 10.1016/j.celrep.2014.03.020 |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Susan Davies |
Date Deposited: | 15 May 2020 15:01 UTC |
Last Modified: | 09 Dec 2022 08:42 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/81269 (The current URI for this page, for reference purposes) |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):