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The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis

Papagiannouli, Fani, Berry, Cameron Wynn, Fuller, Margaret T. (2019) The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis. Stem Cell Reports, 12 (5). pp. 1024-1040. ISSN 2213-6711. (doi:10.1016/j.stemcr.2019.03.008) (KAR id:78955)

Abstract

Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, the functional unit of differentiation. Here we show that cell polarity and vesicle trafficking influence signal transduction in cyst cells, with profound effects on the germ cells they enclose. Our data suggest that the cortical components Dlg, Scrib, Lgl and the clathrin-mediated endocytic (CME) machinery downregulate epidermal growth factor receptor (EGFR) signaling. Knockdown of dlg, scrib, lgl, or CME components in cyst cells resulted in germ cell death, similar to increased signal transduction via the EGFR, while lowering EGFR or downstream signaling components rescued the defects. This work provides insights into how cell polarity and endocytosis cooperate to regulate signal transduction and sculpt developing tissues.

Item Type: Article
DOI/Identification number: 10.1016/j.stemcr.2019.03.008
Divisions: Divisions > Division of Natural Sciences > Kent and Medway Medical School
Depositing User: Fani Papagiannouli
Date Deposited: 22 Nov 2019 14:21 UTC
Last Modified: 04 Mar 2024 19:59 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/78955 (The current URI for this page, for reference purposes)

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