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Consistent responses of the human vascular smooth muscle cell in culture: Implications for restenosis

Munro, E., Chan, P., Patel, M., Betteridge, L., Gallagher, K., Schachter, M., Sever, P., Wolfe, J. (1994) Consistent responses of the human vascular smooth muscle cell in culture: Implications for restenosis. Journal of Vascular Surgery, 20 (3). pp. 482-487. ISSN 0741-5214. (doi:10.1016/0741-5214(94)90149-X) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:78354)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
https://doi.org/10.1016/0741-5214(94)90149-X

Abstract

Purpose: The mechanisms whereby restenoses occur at discrete sites within the vasculature remain uncertain. We have recently reported that vascular smooth muscle cells (VSMC) derived from patients with graft stenoses are resistant to growth inhibition by heparin. In this study, we have examined whether VSMC proliferation rates and responses to inhibition by heparin vary according to the individual or the anatomic site of origin. Methods: Long saphenous veins from seven patients were divided into proximal, middle, and distal portions, and VSMC were cultured separately from each. VSMC proliferation in response to 15% fetal calf serum ± 100 μg/ml heparin was measured by counting triplicate samples at 0, 3, 7, 10, and 14 days. This experiment was repeated from the second to the sixth passage (n = 6) and for artery and vein pairs derived from four additional patients. Results: Differences between vein segment cultures of individual veins were found not to differ significantly from experimental error for either proliferation or heparin inhibition and were not altered by repeated passage (ANOVA). There were, however, significant differences in sensitivity to heparin inhibition between patients (p = 0.02) (ANOVA). There were no significant differences between paired samples of artery and vein for either proliferation or heparin inhibition (Mann-Whitney test). Conclusions: VSMC growth characteristics reflect the individual patient and are maintained in cell culture.

Item Type: Article
DOI/Identification number: 10.1016/0741-5214(94)90149-X
Uncontrolled keywords: amphotericin b, heparin, kanamycin, penicillin g, streptomycin, angioplasty, article, atherectomy, cell hyperplasia, cell proliferation, clinical article, controlled study, coronary artery bypass graft, dose response, graft failure, growth regulation, human, human cell, human tissue, pathogenesis, postoperative complication, priority journal, saphenous vein, smooth muscle fiber, Analysis of Variance, Arteries, Cell Division, Cells, Cultured, Comparative Study, Graft Occlusion, Vascular, Growth Inhibitors, Heparin, Human, Muscle, Smooth, Vascular, Recurrence, Saphenous Vein, Sensitivity and Specificity, Support, Non-U.S. Gov't, Tissue Culture
Divisions: Divisions > Division of Natural Sciences > Kent and Medway Medical School
Depositing User: Philip Chan
Date Deposited: 07 Nov 2019 16:35 UTC
Last Modified: 14 Dec 2023 04:15 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/78354 (The current URI for this page, for reference purposes)

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