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TORC2-Gad8 dependent myosin phosphorylation modulates regulation by calcium

Baker, Karen, Gyamfi, Irene A., Mashanov, Gregory I., Molloy, Justin E., Geeves, Michael A., Mulvihill, Daniel P. (2019) TORC2-Gad8 dependent myosin phosphorylation modulates regulation by calcium. eLife, 8 . E-ISSN 2050-084X. (doi:10.7554/eLife.51150) (KAR id:76937)

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Abstract

Cells respond to changes in their environment through signalling networks that modulate cytoskeleton and membrane organisation to coordinate cell cycle progression, polarised cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission yeast type 1 myosin, Myo1, is modulated by TORC2 signalling dependent phosphorylation. Phosphorylation of the conserved serine at position 742 within the neck region changes both the conformation of the neck region and the interactions between Myo1 and its associating calmodulin light chains. S742 phosphorylation thereby couples calcium and TOR signalling networks in the modulation of myosin-1 dynamics to co-ordinate actin polymerisation and membrane reorganisation at sites of endocytosis and polarised cell growth in response to environmental and cell cycle cues.

Item Type: Article
DOI/Identification number: 10.7554/eLife.51150
Uncontrolled keywords: pombe, endocytosis, calmodulin, Tor kinase
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Daniel Mulvihill
Date Deposited: 01 Oct 2019 09:35 UTC
Last Modified: 31 Jul 2020 15:19 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/76937 (The current URI for this page, for reference purposes)
Geeves, Michael A.: https://orcid.org/0000-0002-9364-8898
Mulvihill, Daniel P.: https://orcid.org/0000-0003-2502-5274
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