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Examination of the expression of immunity genes and bacterial profiles in the caecum of growing chickens infected with Salmonella Enteritidis and fed a phytobiotic

Laptev, Georgi Yu, Filippova, Valentina A., Kochish, Ivan I., Yildirim, Elena A., Ilina, Larisa A., Dubrovin, Andrei V., Brazhnik, Evgeni A., Novikova, Natalia I., Novikova, Oksana B., Dmitrieva, Margarita E., and others. (2019) Examination of the expression of immunity genes and bacterial profiles in the caecum of growing chickens infected with Salmonella Enteritidis and fed a phytobiotic. Animals, 9 (9). Article Number 615. ISSN 2076-2615. (doi:10.3390/ani9090615) (KAR id:75995)

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https://dx.doi.org/10.3390/ani9090615

Abstract

This study was performed to investigate the differential expression of eight immunity genes and the bacterial profiles in the caecum of growing chickens challenged with Salmonella enterica serovar Enteritidis (SE) at 1 and 23 days post inoculation (dpi) in response to SE infection at 19 days of age and administration of the phytobiotic Intebio. Following infection, the genes CASP6 and IRF7 were upregulated by greater than twofold. Chicks fed Intebio showed at 1 dpi upregulation of AvBD10, IL6, IL8L2, CASP6 and IRF7. At 23 dpi, expression of AvBD11, IL6, IL8L2, CASP6 and IRF7 lowered in the experiment subgroups as compared with the control. Examination of the caecal contents at 1 dpi demonstrated a significant decrease in the microbial biodiversity in the infected subgroup fed normal diet. Bacterial content of Lactobacillus and Bacillus declined, while that of Enterobacteriaceae rose. In the infected subgroup fed Intebio, a pronounced change in composition of the microflora was not observed. In the early infection stages, the phytobiotic seemed to promote response to infection. Subsequently, an earlier suppression of the inflammatory reaction took place in chickens fed Intebio. Thus, use of Intebio as a drug with phytobiotic activity in chickens, including those infected with Salmonella, proved to be promising.

Item Type: Article
DOI/Identification number: 10.3390/ani9090615
Projects: [UNSPECIFIED] Development of State-of-the-Art Biotechnologies to Assess Gene Expression in Relation to Performance and Disease Resistance in Poultry Industry
Uncontrolled keywords: gene expression; real-time qPCR; Salmonella; infection; phytobiotic; caecum; microbiome; T-RFLP; poultry; chickens
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
S Agriculture > SF Animal culture
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: Mike Romanov
Date Deposited: 27 Aug 2019 16:45 UTC
Last Modified: 21 Sep 2020 15:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/75995 (The current URI for this page, for reference purposes)
Laptev, Georgi Yu: https://orcid.org/0000-0002-8795-6659
Filippova, Valentina A.: https://orcid.org/0000-0001-8789-9837
Kochish, Ivan I.: https://orcid.org/0000-0002-8502-6052
Yildirim, Elena A.: https://orcid.org/0000-0002-5846-5105
Ilina, Larisa A.: https://orcid.org/0000-0003-2789-4844
Dubrovin, Andrei V.: https://orcid.org/0000-0001-8424-4114
Brazhnik, Evgeni A.: https://orcid.org/0000-0003-2178-9330
Novikova, Natalia I.: https://orcid.org/0000-0002-9647-4184
Novikova, Oksana B.: https://orcid.org/0000-0003-0046-625X
Surai, Peter F.: https://orcid.org/0000-0002-5012-8681
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
Romanov, Michael N.: https://orcid.org/0000-0003-3584-4644
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