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Genetic tool development in marine protists: Emerging model organisms for experimental cell biology

Faktorová, Drahomíra, Nisbet, Ellen R., Fernández Robledo, José A., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares Jr., Manuel, Aresté, Christina, Balestreri, Cecilia, Barbrook, Adrian C., and others. (2020) Genetic tool development in marine protists: Emerging model organisms for experimental cell biology. Nature Methods, . ISSN 1548-7091. E-ISSN 1548-7105. (doi:10.1101/718239doi) (KAR id:75869)

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Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Item Type: Article
DOI/Identification number: 10.1101/718239doi
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Anastasios Tsaousis
Date Deposited: 19 Aug 2019 12:52 UTC
Last Modified: 20 Apr 2020 09:34 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/75869 (The current URI for this page, for reference purposes)
von der Haar, Tobias: https://orcid.org/0000-0002-6031-9254
Tsaousis, Anastasios D: https://orcid.org/0000-0002-5424-1905
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