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Genetic tool development in marine protists: Emerging model organisms for experimental cell biology

Faktorová, Drahomíra, Nisbet, Ellen R., Fernández Robledo, José A., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares Jr., Manuel, Aresté, Christina, Balestreri, Cecilia, Barbrook, Adrian C., and others. (2019) Genetic tool development in marine protists: Emerging model organisms for experimental cell biology. Nature Methods, . ISSN 1548-7091. E-ISSN 1548-7105. (Submitted) (doi:10.1101/718239doi)

Abstract

Marine microbial eukaryotes underpin the largest food web on the planet and influence global biogeochemical cycles that maintain habitability. They are also remarkably diverse and provide insights into evolution, including the origins of complex life forms, as revealed through genome analyses. However, their genetic tractability has been limited to a few species that do not represent the broader diversity of eukaryotic life or some of the most environmentally relevant taxa. Here, we report on genetic systems developed as a community resource for experimental cell biology of marine protists from across the eukaryotic tree of life. We outline DNA delivery methods, expression constructs, and genome editing approaches that proved successful, as well as results from taxa where a working system was not achieved. The reported breakthroughs on genetic manipulation position the community to dissect cellular mechanisms from a breadth of protists, which will collectively provide insights into ancestral eukaryotic lifeforms, protein diversification and evolution of cellular pathways.

Item Type: Article
DOI/Identification number: 10.1101/718239doi
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Anastasios Tsaousis
Date Deposited: 19 Aug 2019 12:52 UTC
Last Modified: 04 Dec 2019 14:22 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/75869 (The current URI for this page, for reference purposes)
von der Haar, Tobias: https://orcid.org/0000-0002-6031-9254
Tsaousis, Anastasios D: https://orcid.org/0000-0002-5424-1905
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