Faktorová, Drahomíra, Nisbet, Ellen R., Fernández Robledo, José A., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares Jr., Manuel, Aresté, Christina, Balestreri, Cecilia, Barbrook, Adrian C., and others. (2020) Genetic tool development in marine protists: Emerging model organisms for experimental cell biology. Nature Methods, . ISSN 1548-7091. E-ISSN 1548-7105. (doi:10.1101/718239doi) (KAR id:75869)
|
PDF
Publisher pdf
Language: English 
This work is licensed under a Creative Commons Attribution 4.0 International License.
|
|
|
Download this file (PDF/7MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
|
PDF
Pre-print
Language: English 
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
|
|
|
Download this file (PDF/12MB) |
Preview |
| Request a format suitable for use with assistive technology e.g. a screenreader | |
| Official URL: http://dx.doi.org/10.1101/718239doi |
|
Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1101/718239doi |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Anastasios Tsaousis |
| Date Deposited: | 19 Aug 2019 12:52 UTC |
| Last Modified: | 05 Nov 2024 12:40 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/75869 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Kazana, Eleanna.
| Creator's ORCID: | |
|---|---|
| CReDIT Contributor Roles: |
von der Haar, Tobias.
| Creator's ORCID: |
 https://orcid.org/0000-0002-6031-9254
|
|---|---|
| CReDIT Contributor Roles: |
Tsaousis, Anastasios D.
| Creator's ORCID: |
https://orcid.org/0000-0002-5424-1905
|
|---|---|
| CReDIT Contributor Roles: |
- Link to SensusAccess
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric
