Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes

Brandao, Pedro F. B. and Bull, Alan T. (2003) Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes. Antonie Van Leeuwenhoek, 84 (2). pp. 89-98. ISSN 0003-6072. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1023/A:1025409818275

Abstract

Nitrile metabolising actinomycetes previously recovered from deep-sea sediments and terrestrial soils were investigated for their nitrile transforming properties. Metabolic profiling and activity assays confirmed that all strains catalysed the hydrolysis of nitriles by a nitrile hydratase/amidase system. Acetonitrile and benzonitrile, when used as growth substrates for enzyme induction experiments, had a significant influence on the biotransformation activities towards various nitriles and amides. The specific activities of selected deep-sea and terrestrial acetonitrile-grown bacteria against a suite of nitriles and amides were higher than those of the only other reported marine nitrile-hydrolysing R. erythropolis, isolated from a shallow sediment. The increase of nitrile chain length appeared to have negative influence on the nitrile hydratase activity of acetonitrile-grown bacteria, but the same was not true for benzonitrile-grown bacteria. The nitrile hydratases and amidases were constitutive in 10 of the 16 deep-sea and terrestrial actinomycetes studied, and one strain showed an inducible hydratase and a constitutive amidase. Most of the deep-sea strains had constitutive activities and showed some of the highest activities and broadest substrate specificities of organisms included in this study.

Item Type: Article
Additional information: 0003-6072 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Acetamides/metabolism Acetonitriles/metabolism Actinobacteria/growth & development/isolation & purification/*metabolism Amidohydrolases/metabolism Biotransformation Geologic Sediments/microbiology Hydro-Lyases/metabolism Hydrolysis Kinetics Nitriles/*metabolism Rhodococcus/growth & development/isolation & purification/metabolism Seawater/microbiology Soil Microbiology Substrate Specificity
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 09 Sep 2008 08:57
Last Modified: 22 Apr 2014 11:37
Resource URI: https://kar.kent.ac.uk/id/eprint/7351 (The current URI for this page, for reference purposes)
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