Brandao, Pedro F. B., Bull, Alan T. (2003) Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes. Antonie Van Leeuwenhoek, 84 (2). pp. 89-98. ISSN 0003-6072. (doi:10.1023/A:1025409818275) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:7351)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1023/A:1025409818275 |
Abstract
Nitrile metabolising actinomycetes previously recovered from deep-sea sediments and terrestrial soils were investigated for their nitrile transforming properties. Metabolic profiling and activity assays confirmed that all strains catalysed the hydrolysis of nitriles by a nitrile hydratase/amidase system. Acetonitrile and benzonitrile, when used as growth substrates for enzyme induction experiments, had a significant influence on the biotransformation activities towards various nitriles and amides. The specific activities of selected deep-sea and terrestrial acetonitrile-grown bacteria against a suite of nitriles and amides were higher than those of the only other reported marine nitrile-hydrolysing R. erythropolis, isolated from a shallow sediment. The increase of nitrile chain length appeared to have negative influence on the nitrile hydratase activity of acetonitrile-grown bacteria, but the same was not true for benzonitrile-grown bacteria. The nitrile hydratases and amidases were constitutive in 10 of the 16 deep-sea and terrestrial actinomycetes studied, and one strain showed an inducible hydratase and a constitutive amidase. Most of the deep-sea strains had constitutive activities and showed some of the highest activities and broadest substrate specificities of organisms included in this study.
Item Type: | Article |
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DOI/Identification number: | 10.1023/A:1025409818275 |
Additional information: | 0003-6072 (Print) Journal Article Research Support, Non-U.S. Gov't |
Uncontrolled keywords: | Acetamides/metabolism Acetonitriles/metabolism Actinobacteria/growth & development/isolation & purification/*metabolism Amidohydrolases/metabolism Biotransformation Geologic Sediments/microbiology Hydro-Lyases/metabolism Hydrolysis Kinetics Nitriles/*metabolism Rhodococcus/growth & development/isolation & purification/metabolism Seawater/microbiology Soil Microbiology Substrate Specificity |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Susan Davies |
Date Deposited: | 09 Sep 2008 08:57 UTC |
Last Modified: | 05 Nov 2024 09:39 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/7351 (The current URI for this page, for reference purposes) |
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