Automated nuclear cartography reveals conserved sperm chromosome territory localization across 2 million years of mouse evolution

Skinner, Benjamin M., Bacon, Joanne, Rathje, Claudia C., Larson, Erica L., Kopania, Emily E. K., Good, Jeffrey M., Affara, Nabeel A., Ellis, Peter J.I. (2019) Automated nuclear cartography reveals conserved sperm chromosome territory localization across 2 million years of mouse evolution. Genes, 10 (2). Article Number 109. (doi:10.3390/genes10020109) (KAR id:72162)

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Official URL https://doi.org/10.3390/genes10020109

Abstract

Measurements of nuclear organization in asymmetric nuclei in 2D images have

sperm samples, typically by dividing the nucleus into zones, and manually scoring which zone a

prone to subjectivity. We have developed a new approach for automated mapping of FISH signals

Automatic landmark detection defines equivalent structural regions in each nucleus, then dynamic

within the entire cell population. Using this approach, we mapped the positions of the sex

musculus and Mus spretus). We found that in all three, chromosomes 11 and 19 tend to interact with

conserved across 2 million years of mouse evolution.

Item Type:	Article
DOI/Identification number:	10.3390/genes10020109
Projects:	[UNSPECIFIED] Customisable surface markers for the separation of X and Y bearing mammalian sperm
Uncontrolled keywords:	sperm, quantitative morphometry, morphometrics, fertility, evolution, image analysis
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QH Natural history > QH581.2 Cell Biology
Divisions:	Divisions > Division of Natural Sciences > School of Biosciences
Depositing User:	Peter Ellis
Date Deposited:	05 Feb 2019 10:27 UTC
Last Modified:	05 Oct 2020 14:06 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/72162 (The current URI for this page, for reference purposes)
Skinner, Benjamin M.:	https://orcid.org/0000-0002-7152-1167
Ellis, Peter J.I.:	https://orcid.org/0000-0001-9709-7934

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