Skinner, Benjamin M., Bacon, Joanne, Rathje, Claudia C., Larson, Erica L., Kopania, Emily E. K., Good, Jeffrey M., Affara, Nabeel A., Ellis, Peter J.I. (2019) Automated nuclear cartography reveals conserved sperm chromosome territory localization across 2 million years of mouse evolution. Genes, 10 (2). 109;. (doi:10.3390/genes10020109) (KAR id:72162)
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| Official URL https://doi.org/10.3390/genes10020109 |
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Abstract
Measurements of nuclear organization in asymmetric nuclei in 2D images have
sperm samples, typically by dividing the nucleus into zones, and manually scoring which zone a
prone to subjectivity. We have developed a new approach for automated mapping of FISH signals
Automatic landmark detection defines equivalent structural regions in each nucleus, then dynamic
within the entire cell population. Using this approach, we mapped the positions of the sex
musculus and Mus spretus). We found that in all three, chromosomes 11 and 19 tend to interact with
conserved across 2 million years of mouse evolution.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.3390/genes10020109 |
| Projects: | [UNSPECIFIED] Customisable surface markers for the separation of X and Y bearing mammalian sperm |
| Uncontrolled keywords: | sperm, quantitative morphometry, morphometrics, fertility, evolution, image analysis |
| Subjects: |
Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QH Natural history > QH581.2 Cell Biology |
| Divisions: | Faculties > Sciences > School of Biosciences |
| Depositing User: | Peter Ellis |
| Date Deposited: | 05 Feb 2019 10:27 UTC |
| Last Modified: | 30 May 2019 08:52 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/72162 (The current URI for this page, for reference purposes) |
| Skinner, Benjamin M.: |  https://orcid.org/0000-0002-7152-1167 |
| Ellis, Peter J.I.: | https://orcid.org/0000-0001-9709-7934 |
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