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A high-throughput method for unbiased quantitation and categorisation of nuclear morphology

Skinner, Benjamin M., Rathje, Claudia C., Bacon, Joanne, Johnson, Emma P. S., Larson, Erica L., Kopania, Emily E. K., Good, Jeffrey M., Yousafzai, G., Affara, Nabeel A., Ellis, Peter J.I. and others. (2019) A high-throughput method for unbiased quantitation and categorisation of nuclear morphology. Biology of Reproduction, 100 (5). pp. 1250-1260. ISSN 0006-3363. (doi:10.1093/biolre/ioz013)

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Abstract

The physical arrangement of chromatin in the nucleus is cell type and species specific; a fact

characteristic falciform (‘hook shaped’) sperm in mice is important in studies of sperm

shape differences typically relies on subjective manual assessment, rendering comparisons

and characterised the sperm of mice from a range of inbred, outbred and wild-derived

within and between samples in comparison to wild-derived inbred strains, and that sperm

the direction of the cross. We further show that hierarchical clustering can discriminate

manual assessors, and is useful both to distinguish between samples and also to identify

scale and will be widely applicable to different species and different areas of biology.

Item Type: Article
DOI/Identification number: 10.1093/biolre/ioz013
Projects: [UNSPECIFIED] Customisable surface markers for the separation of X and Y bearing mammalian sperm
Uncontrolled keywords: sperm, quantitative morphometry, morphometrics, fertility, evolution, image analysis
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH581.2 Cell Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Peter Ellis
Date Deposited: 05 Feb 2019 10:19 UTC
Last Modified: 07 Jun 2019 14:28 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72160 (The current URI for this page, for reference purposes)
Skinner, Benjamin M.: https://orcid.org/0000-0002-7152-1167
Ellis, Peter J.I.: https://orcid.org/0000-0001-9709-7934
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