Levison, P.R., Badger, S.E., Hathi, P., Davies, M.J., Bruce, Ian J., Grimm, V. (1998) New approaches to the isolation of DNA by ion-exchange chromatography. Journal of Chromatography A, 827 (2). pp. 337-344. ISSN 0021-9673. (doi:10.1016/S0021-9673(98)00681-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71974)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1016/S0021-9673(98)00681-5 |
Abstract
The performance of different anion-exchange media have been compared for the isolation of plasmid DNA and genomic DNA from bacterial cells and human whole blood. Whatman DEAE-Magarose, based on an agarose bead containing a paramagnetic component, has been compared with prepacked gravity-flow columns containing a derivatised silica matrix. In each case the DNA isolation at various scales of operation was similar both in terms of yield and quality. The magnetic susceptibility of DEAE-Magarose is very high, facilitating the use of this separation technique for rapid flexible batch chromatographic processes, a limitation of the prepacked column techniques.
Item Type: | Article |
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DOI/Identification number: | 10.1016/S0021-9673(98)00681-5 |
Uncontrolled keywords: | plasmid DNA, adsorption; blood; centrifugation; conference paper; DNA determination; escherichia coli; genome; human; ion exchange chromatography; nonhuman; priority journal, Chromatography, Ion Exchange; DNA; DNA, Bacterial; Electrophoresis, Agar Gel; Escherichia coli; Humans, Bacteria (microorganisms) |
Divisions: | Divisions > Division of Natural Sciences > Physics and Astronomy |
Funders: | [UNSPECIFIED] EPSRC-DTI |
Depositing User: | Ian Bruce |
Date Deposited: | 30 Jan 2019 18:18 UTC |
Last Modified: | 16 Nov 2021 10:26 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/71974 (The current URI for this page, for reference purposes) |
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