New approaches to the isolation of DNA by ion-exchange chromatography

Levison, P.R., Badger, S.E., Hathi, P., Davies, M.J., Bruce, Ian J., Grimm, V. (1998) New approaches to the isolation of DNA by ion-exchange chromatography. Journal of Chromatography A, 827 (2). pp. 337-344. ISSN 0021-9673. (doi:10.1016/S0021-9673(98)00681-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71974)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1016/S0021-9673(98)00681-5

Abstract

The performance of different anion-exchange media have been compared for the isolation of plasmid DNA and genomic DNA from bacterial cells and human whole blood. Whatman DEAE-Magarose, based on an agarose bead containing a paramagnetic component, has been compared with prepacked gravity-flow columns containing a derivatised silica matrix. In each case the DNA isolation at various scales of operation was similar both in terms of yield and quality. The magnetic susceptibility of DEAE-Magarose is very high, facilitating the use of this separation technique for rapid flexible batch chromatographic processes, a limitation of the prepacked column techniques.

Item Type:	Article
DOI/Identification number:	10.1016/S0021-9673(98)00681-5
Uncontrolled keywords:	plasmid DNA, adsorption; blood; centrifugation; conference paper; DNA determination; escherichia coli; genome; human; ion exchange chromatography; nonhuman; priority journal, Chromatography, Ion Exchange; DNA; DNA, Bacterial; Electrophoresis, Agar Gel; Escherichia coli; Humans, Bacteria (microorganisms)
Institutional Unit:	Schools > School of Engineering, Mathematics and Physics > Physics and Astronomy
Former Institutional Unit:	Divisions > Division of Natural Sciences > Physics and Astronomy
Funders:	DTI University (https://ror.org/00xk3ch77)
Depositing User:	Ian Bruce
Date Deposited:	30 Jan 2019 18:18 UTC
Last Modified:	20 May 2025 09:44 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/71974 (The current URI for this page, for reference purposes)

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Bruce, Ian J..

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