East, D.A. and Todd, M. and Bruce, Ian J. (2014) Quantum dot-antibody conjugates via carbodiimide-mediated coupling for cellular imaging. In: Fontes, Adriana and Saegesser Santos, Beate, eds. Quantum Dots: Applications in Biology. Methods in Molecular Biology . Springer, pp. 67-83. ISBN 978-1-4939-1279-7. (doi:10.1007/978-1-4939-1280-3_5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71928)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1007/978-1-4939-1280-3_5 |
Abstract
This chapter describes the processes of antibody (Ab) production, purification, conjugation to quantum dots (QDs), and the use of the conjugates produced in intracellular imaging of cell components and structures. Specifically, information is provided on the conjugation of carboxyl surface-terminated QDs to Abs via a one-step reaction using the water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The chapter details the process of conjugate optimization in terms of its final fluorescence and biological activity. The method described should guarantee the production of QD-Ab conjugates, which outperform classic organic fluorophore-Ab conjugates in terms of both image definition produced and the longevity of the imaging agent.
| Item Type: | Book section |
|---|---|
| DOI/Identification number: | 10.1007/978-1-4939-1280-3_5 |
| Uncontrolled keywords: | 1 (3 dimethylaminopropyl) 3 ethylcarbodiimide; amine; antibody conjugate; carboxyl group; carboxylic acid; cyanamide; quantum dot; antibody; Cdc8 protein, S pombe; cell cycle protein; cyanamide; quantum dot; Schizosaccharomyces pombe protein; water, antibody affinity; antibody production; article; biological activity; cell component; cell structure; conjugation; controlled study; fluorescence; imaging; immunofluorescence; nonhuman; polyacrylamide gel electrophoresis; priority journal; process optimization; purification; ultrafiltration; chemistry; fluorescent antibody technique; intracellular space; isolation and purification; metabolism; molecular imaging; procedures; spectrofluorometry, Amines; Antibodies; Carbodiimides; Cell Cycle Proteins; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; Intracellular Space; Molecular Imaging; Quantum Dots; Schizosaccharomyces pombe Proteins; Spectrometry, Fluorescence; Ultrafiltration; Water |
| Subjects: |
Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD156 Inorganic synthesis |
| Divisions: | Divisions > Division of Natural Sciences > Physics and Astronomy |
| Funders: | EC Systems (Poland) (https://ror.org/03d73se48) |
| Depositing User: | Ian Bruce |
| Date Deposited: | 01 Feb 2019 13:16 UTC |
| Last Modified: | 05 Nov 2024 12:34 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/71928 (The current URI for this page, for reference purposes) |
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