Localization of the Binding Site for the Oligosaccharide Moiety of Gb 3 on Verotoxin 1 Using NMR Residual Dipolar Coupling Measurements

Thompson, GS, Shimizu, H, Homans, SW, Donohue-Rolfe, A (2000) Localization of the Binding Site for the Oligosaccharide Moiety of Gb 3 on Verotoxin 1 Using NMR Residual Dipolar Coupling Measurements. Biochemistry, 39 . pp. 13153-13156. ISSN 0006-2960. (doi:10.1021/bi001394+) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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http://dx.doi.org/10.1021/bi001394+

Abstract

By use of NMR residual dipolar coupling measurements in a dilute liquid-crystalline solvent, the solution structure has been determined of the complex between the oligosaccharide moiety of globotriaosylceramide (Gb3-OS) and the B-subunit homopentamer of verotoxin 1 (VTB). The dipolar coupling data indicate that Gb3-OS binds in a single binding site per monomer, which is identical to one of three sites inferred from the X-ray structure of the same complex. We find no evidence within experimental error for occupancy at either of the two additional binding sites observed per monomer in the crystal structure.

Item Type: Article
DOI/Identification number: 10.1021/bi001394+
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: G. Thompson
Date Deposited: 23 Jan 2019 16:25 UTC
Last Modified: 30 May 2019 08:47 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71826 (The current URI for this page, for reference purposes)
Thompson, GS: https://orcid.org/0000-0001-9399-7636
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