Thompson, GS, Shimizu, H, Homans, SW, Donohue-Rolfe, A (2000) Localization of the Binding Site for the Oligosaccharide Moiety of Gb 3 on Verotoxin 1 Using NMR Residual Dipolar Coupling Measurements. Biochemistry, 39 . pp. 13153-13156. ISSN 0006-2960. (doi:10.1021/bi001394+) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71826)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1021/bi001394+ |
Abstract
By use of NMR residual dipolar coupling measurements in a dilute liquid-crystalline solvent, the solution structure has been determined of the complex between the oligosaccharide moiety of globotriaosylceramide (Gb3-OS) and the B-subunit homopentamer of verotoxin 1 (VTB). The dipolar coupling data indicate that Gb3-OS binds in a single binding site per monomer, which is identical to one of three sites inferred from the X-ray structure of the same complex. We find no evidence within experimental error for occupancy at either of the two additional binding sites observed per monomer in the crystal structure.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1021/bi001394+ |
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Gary Thompson |
| Date Deposited: | 23 Jan 2019 16:25 UTC |
| Last Modified: | 16 Nov 2021 10:26 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/71826 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Thompson, GS.
| Creator's ORCID: |
 https://orcid.org/0000-0001-9399-7636
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|---|---|
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