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Thermoresponsive Polymer Micropatterns Fabricated by Dip-Pen Nanolithography for a Highly Controllable Substrate with Potential Cellular Applications

Laing, Stacey, Suriano, Raffaella, Lamprou, Dimitrios A., Smith, Carol-Anne, Dalby, Matthew J., Mabbott, Samuel, Faulds, Karen, Graham, Duncan (2016) Thermoresponsive Polymer Micropatterns Fabricated by Dip-Pen Nanolithography for a Highly Controllable Substrate with Potential Cellular Applications. ACS Applied Materials & Interfaces, 8 (37). pp. 24844-24852. ISSN 1944-8244. E-ISSN 1944-8252. (doi:10.1021/acsami.6b03860) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanized silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behavior of polymer arrays which swell/deswell in aqueous solution in response to a change in temperature was successfully characterized by atomic force microscopy (AFM) and Raman spectroscopy: a thermally induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behavior when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.

Item Type: Article
DOI/Identification number: 10.1021/acsami.6b03860
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA 403 Materials Science
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Dimitrios Lamprou
Date Deposited: 19 Sep 2017 12:35 UTC
Last Modified: 29 May 2019 19:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/63484 (The current URI for this page, for reference purposes)
Lamprou, Dimitrios A.: https://orcid.org/0000-0002-8740-1661
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