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Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS

Weeks, Mark E., von Caron, G.N., James, David C., Smales, Christopher Mark, Robinson, Gary K. (2006) Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS. Journal of Microbiological Methods, 66 (1). pp. 43-55. ISSN 0167-7012. (doi:10.1016/j.mimet.2005.10.008) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:6227)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1016/j.mimet.2005.10.008

Abstract

Listeria monocytogenes has previously been shown to adapt to a wide variety of environmental niches, principally those associated with low pH, and this compromises its control in food environments. An understanding of the mechanism(s) by which L. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. The present Study aimed to gain a further understanding of the physiological basis for the differential effects of one control strategy, namely the use of the lantibiotic nisin. Using propidium iodide (PI) to probe membrane integrity it was shown that L. monocytogenes Scott A was sensitive to nisin (8 ng mL(-1)) but this was growth phase dependent with stationary phase cells (OD600=1.2) being much more resistant than exponential phase cells (OD600=0.38). We demonstrate that, using a combination of techniques including fluorescence activated cell sorting (FACS), the membrane adaptations underpinning nisin resistance are triggered much earlier (OD600 < 0.5) than the onset of stationary phase. The significance of these findings in terms of mechanism and application are discussed.

Item Type: Article
DOI/Identification number: 10.1016/j.mimet.2005.10.008
Uncontrolled keywords: Listeria monocytogenes; exponential phase; stationary phase; nisin; quorum sensing
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mark Smales
Date Deposited: 03 Sep 2008 07:26 UTC
Last Modified: 16 Nov 2021 09:44 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/6227 (The current URI for this page, for reference purposes)

University of Kent Author Information

James, David C..

Creator's ORCID:
CReDIT Contributor Roles:

Smales, Christopher Mark.

Creator's ORCID: https://orcid.org/0000-0002-2762-4724
CReDIT Contributor Roles:

Robinson, Gary K..

Creator's ORCID: https://orcid.org/0000-0003-2660-7778
CReDIT Contributor Roles:
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