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Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture

Weeks, Mark E., James, David C., Robinson, Gary K., Smales, Christopher Mark (2004) Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture. Proteomics, 4 (1). pp. 123-135. ISSN 1615-9853. (doi:10.1002/pmic.200300527) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:61)

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Abstract

Listeria monocytogenes is a food-borne Gram-positive bacterium that is responsible for a variety of infections (worldwide) annually. The organism is able to survive a variety of environmental conditions and stresses, however, the mechanisms by which L. monocytogenes adapts to environmental change are yet to be fully elucidated. An understanding of the mechanism(s) by which L. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. We have utilized a proteomic approach to investigate the response of L. monocytogenes batch cultures to the transition from exponential to stationary growth phase. Proteomic analysis showed that batch cultures of L. monocytogenes perceived stress and began preparations for stationary phase much earlier (approximately A(600) = 0.75, mid-exponential) than predicted by growth characteristics alone. Global analysis of the proteome revealed that the expression levels of more than 50% of all proteins observed changed significantly over a 7-9 h period during this transition phase. We have highlighted ten proteins in particular whose expression levels appear to be important in the early onset of the stationary phase. The significance of these findings in terms of functionality and the mechanistic picture are discussed.

Item Type: Article
DOI/Identification number: 10.1002/pmic.200300527
Uncontrolled keywords: environmental adaptation; growth phase; Listeria monocytogenes; two-dimensional gel electrophoresis
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mark Smales
Date Deposited: 19 Dec 2007 17:51 UTC
Last Modified: 16 Nov 2021 09:38 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61 (The current URI for this page, for reference purposes)

University of Kent Author Information

James, David C..

Creator's ORCID:
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Robinson, Gary K..

Creator's ORCID: https://orcid.org/0000-0003-2660-7778
CReDIT Contributor Roles:

Smales, Christopher Mark.

Creator's ORCID: https://orcid.org/0000-0002-2762-4724
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