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Plasma and saliva miR-21 expression in colorectal cancer patients

Sazanov, Alexei A, Kiselyova, E V, Zakharenko, A A, Romanov, Michael N, Zaraysky, M I (2017) Plasma and saliva miR-21 expression in colorectal cancer patients. Journal of Applied Genetics, 58 (2). pp. 231-237. ISSN 1234-1983. E-ISSN 2190-3883. (doi:10.1007/s13353-016-0379-9) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P?=?0.0001; saliva, P?=?5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II–IV as compared to the control group. No correlation of miR-21 expression was found with regard to gender and age of patents. Also, there were no significant individual correlations and linear regression of miR-21 expression in the plasma and saliva. The estimated diagnostic sensitivity and specificity of miR-21 expression were respectively 65 and 85% in the plasma, and 97 and 91% in the saliva. Our data suggest that miR-21 in both the saliva and plasma could be a proper biomarker for CRC screening, although the saliva miR-21 expression test looks preferable due to its higher sensitivity, specificity, and technical simplicity.

Item Type: Article
DOI/Identification number: 10.1007/s13353-016-0379-9
Uncontrolled keywords: Blood plasma, Colorectal cancer, Epigenetics, Gene expression profiling, microRNA (miR), miR-21, Molecular diagnostics, Saliva, Real-time qRT-PCR
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: M. Romanov
Date Deposited: 15 Dec 2016 21:45 UTC
Last Modified: 29 May 2019 18:29 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/59719 (The current URI for this page, for reference purposes)
Romanov, Michael N: https://orcid.org/0000-0003-3584-4644
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