Zfy genes are required for efficient meiotic sex chromosome inactivation (MSCI) in spermatocytes

Vernet, Nadège, Mahadevaiah, Shantha K, de Rooij, Dirk G, Burgoyne, Paul S, Ellis, Peter J.I. (2016) Zfy genes are required for efficient meiotic sex chromosome inactivation (MSCI) in spermatocytes. Human molecular genetics, . ISSN 1460-2083. (doi:10.1093/hmg/ddw344)

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Abstract

During spermatogenesis, germ cells that fail to synapse their chromosomes or fail to undergo meiotic sex chromosome inactivation (MSCI) are eliminated via apoptosis during mid-pachytene. Previous work showed that Y-linked genes Zfy1 and Zfy2 act as "executioners" for this checkpoint, and that wrongful expression of either gene during pachytene triggers germ cell death. Here, we show that in mice, Zfy genes are also necessary for efficient MSCI and the sex chromosomes are not correctly silenced in Zfy-deficient spermatocytes. This unexpectedly reveals a triple role for Zfy at the mid-pachytene checkpoint in which Zfy genes first promote MSCI, then monitor its progress (since if MSCI is achieved, Zfy genes will be silenced), and finally execute cells with MSCI failure. This potentially constitutes a negative feedback loop governing this critical checkpoint mechanism.

Item Type: Article
DOI/Identification number: 10.1093/hmg/ddw344
Subjects: Q Science
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Peter Ellis
Date Deposited: 31 Oct 2016 12:09 UTC
Last Modified: 29 May 2019 18:06 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/58282 (The current URI for this page, for reference purposes)
Vernet, Nadège: https://orcid.org/0000-0002-1308-2469
Ellis, Peter J.I.: https://orcid.org/0000-0001-9709-7934
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