Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans

O'Sullivan, Justin M. and Mihr, Marian J. and Santos, Manuel and Tuite, Mick F. (2001) Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans. Yeast, 18 (4). pp. 313-322. ISSN 0749-503X. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not available from this repository. (Contact us about this Publication)
Official URL<...


A number of Candida species translate the standard leucine-CUG codon as serine using a novel ser-tRNA(CAG). This tRNA, which has an unusual anticodon stem-loop structure, has been implicated in the evolution of this codon reassignment. However, such a sense codon reassignment might also require a change in the specificity of the cognate aminoacyl tRNA-synthetase, in this case the ser-tRNA synthetase. Here we describe the cloning and sequence analysis of the C. albicans seryl aminoacyl-tRNA synthetase (CaSerRS) gene (CaSES1). The predicted CaSerRS sequence shows a significant level of amino acid identity to SerRs from other organisms and fully complements a S. cerevisiae SerRS null strain without any apparent defect in growth rate. This suggests that the SerRS recognizes and charges S. cerevisiae ser-tRNAs with similar efficiency to that of the S. cerevisiae SerRS. Using an antibody raised against CaSerRS, we also demonstrate the presence of SerRS in a range of Candida spp. showing CUG codon reassignment. We conclude that the key element in CUG reassigment in Candida spp. is the tRNA that decodes the CUG codon rather than a SerRS structural change. The nucleotide sequence of the CaSES1 gene has been deposited at GenBank under Accession No. AF290915.

Item Type: Article
Additional information: 0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Candida albicans/*genetics Cloning, Molecular Evolution, Molecular Gene Expression *Genetic Code Genetic Complementation Test Molecular Sequence Data Protein Biosynthesis/*genetics RNA, Transfer/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/*genetics Serine-tRNA Ligase/*genetics/metabolism
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Mick Tuite
Date Deposited: 28 Oct 2008 18:47
Last Modified: 10 Jun 2014 11:22
Resource URI: (The current URI for this page, for reference purposes)
  • Depositors only (login required):