O'Sullivan, Justin M., Mihr, Marian J., Santos, Manuel, Tuite, Mick F. (2001) Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans. Yeast, 18 (4). pp. 313-322. ISSN 0749-503X. (doi:10.1002/1097-0061(20010315)18:4<313::AID-YEA673>3.0.CO;2-7) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:5466)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1002/1097-0061(20010315)18:4<... |
Abstract
A number of Candida species translate the standard leucine-CUG codon as serine using a novel ser-tRNA(CAG). This tRNA, which has an unusual anticodon stem-loop structure, has been implicated in the evolution of this codon reassignment. However, such a sense codon reassignment might also require a change in the specificity of the cognate aminoacyl tRNA-synthetase, in this case the ser-tRNA synthetase. Here we describe the cloning and sequence analysis of the C. albicans seryl aminoacyl-tRNA synthetase (CaSerRS) gene (CaSES1). The predicted CaSerRS sequence shows a significant level of amino acid identity to SerRs from other organisms and fully complements a S. cerevisiae SerRS null strain without any apparent defect in growth rate. This suggests that the SerRS recognizes and charges S. cerevisiae ser-tRNAs with similar efficiency to that of the S. cerevisiae SerRS. Using an antibody raised against CaSerRS, we also demonstrate the presence of SerRS in a range of Candida spp. showing CUG codon reassignment. We conclude that the key element in CUG reassigment in Candida spp. is the tRNA that decodes the CUG codon rather than a SerRS structural change. The nucleotide sequence of the CaSES1 gene has been deposited at GenBank under Accession No. AF290915.
Item Type: | Article |
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DOI/Identification number: | 10.1002/1097-0061(20010315)18:4<313::AID-YEA673>3.0.CO;2-7 |
Additional information: | 0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't |
Uncontrolled keywords: | Amino Acid Sequence Candida albicans/*genetics Cloning, Molecular Evolution, Molecular Gene Expression *Genetic Code Genetic Complementation Test Molecular Sequence Data Protein Biosynthesis/*genetics RNA, Transfer/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/*genetics Serine-tRNA Ligase/*genetics/metabolism |
Subjects: | Q Science > QR Microbiology |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Michael Tuite |
Date Deposited: | 28 Oct 2008 18:47 UTC |
Last Modified: | 05 Nov 2024 09:37 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/5466 (The current URI for this page, for reference purposes) |
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