Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP)

Williamson, Richard A., Marston, Fiona A., Angal, Sarojani, Koklitis, P., Panico, M., Morris, Howard R., Carne, A.F., Smith, B.J., Harris, T.J., Freedman, Robert B. and others. (1990) Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP). Biochemical Journal, 268 (2). pp. 267-274. ISSN 0264-6021. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:5321)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
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Abstract

Disulphide bonds in human recombinant tissue inhibitor of metalloproteinases (TIMP) were assigned by resolving proteolytic digests of TIMP on reverse-phase h.p.l.c. and sequencing those peaks judged to contain disulphide bonds by virtue of a change in retention time on reduction. This procedure allowed the direct assignment of Cys-145-Cys-166 and the isolation of two other peptides containing two disulphide bonds each. Further peptide cleavage in conjunction with fast-atom-bombardment m.s. analysis permitted the assignments Cys-1-Cys-70, Cys-3-Cys-99, Cys-13-Cys-124 and Cys-127-Cys-174 from these peptides. The sixth bond Cys-132-Cys-137 was assigned by inference, as the native protein has no detectable free thiol groups.

Item Type:	Article
Additional information:	0264-6021 (Print) Journal Article
Uncontrolled keywords:	Amino Acid Sequence Animals Cell Line Chromatography, High Pressure Liquid DNA/analysis Disulfides/analysis Glycoproteins Humans Hydrolysis Metalloendopeptidases Mice *Microbial Collagenase/antagonists & inhibitors Molecular Sequence Data Pepsin A Recombinant Proteins/genetics Tissue Inhibitor of Metalloproteinases Trypsin
Subjects:	Q Science
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Richard Williamson
Date Deposited:	03 Oct 2009 09:24 UTC
Last Modified:	16 Nov 2021 09:43 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/5321 (The current URI for this page, for reference purposes)

University of Kent Author Information

Williamson, Richard A..

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Freedman, Robert B..

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